Objective      To investigate the relationship of single nucleotide polymorphism (SNP) of AMPKɑ2 gene rs1124900G/T with type 2 diabetes (T2DM)

serum adiponectin (APN) level and carotid atherosclerosis.       Methods      One hundred and seventy-eight patients with T2DM [male 93 and female 85

average age (54.6±10.1)] who hospitalized in the Department of Endocrinology

Second Hospital of Lanzhou University from April 2010 to September 2010 were assigned as a T2DM group

and 160 healthy examinees [male 84 and female 76

average age (51.8±9.8)] at the same period were assigned as a control group. The genotype of AMPKɑ2 rs1124900G/T was examined in all the subjects by PCR-RFLP. The differences of genotype and allele frequency between the two groups were compared. The indices including height

body weight

blood pressure

blood lipid

APN and carotid intima-media thickness (CIMT) were measured.       Results      (1)The genotype frequencies of AMPKɑ2-rs1124900 G/G

G/T and T/T were 29.8%

43.2% and 26.9% in the T2DM group

respectively

and 32.5%

42.5% and 25.0% in the control group

respectively. The T allele frequencies were 48.6% in the T2DM group and 46.3% in the control group. There was no significant difference between the 2 groups (P0.05). (2) In the T2DM group and the control group

the serum levels of TG and LDL-C

CIMT and incidence of plaque in the T/T subgroup were higher than those in the G/G subgroup. The level of APN in the T/T subgroup was significantly lower than that in the G/G group (P0.05). (3) The CIMT and incidence of plaque were positively correlated with the serum levels of TG (r=0.349 6

P0.01) and LDL-C (r=0.416 7

P0.01)

but were negatively correlated with the level of APN (r=-0.262 3

P0.05).       Conclusion      AMPKɑ2-rs1124900 SNP is not related to the incidence of T2DM in Gansu Province

China

but is related to the serum levels of APN

TG and LDL-C

CIMT and plaque incidence.