Objective      To prepare ultrasound microbubbles (MBs) which can simultaneously load two anticancer drugs and investigate the drug-loading capacity and controlled-release characteristics.       Methods        MBs were fabricated by mechanical oscillation. Paclitaxel (PTX) was embedded into the membrane of MBs

and liposomes loaded with doxorubicin (DOX) were connected with MBs by biotin avidin linker system. The properties of prepared MBs were analyzed for their size by particle size analyzer and for their surface features by fluorescent microscopy. UV-visible spectrometry and multi-function microplate reader were used to i

dentify their loaded doses of PTX and DOX respectively. The efficiency of ultrasound mediated drug release were identified by measuring the amount of PTX and DOX releasing from MBs.       Results       The prepared MBs were in an average diameter of (1.45±0.27) μm with red fluorescent surrounding. When 3 mg phospholipid was given

the maximum of PTX encapsulation efficiency was (54.64±2.98)% and each 1×10

8

microbubbles containing (4.52±0.53) μg DOX. When ultrasonic applications had the mechanical index of 1.0 (MI=1.0)

the destruction of the MBs was 90% and the efficiency of PTX and DOX releasing were 15% and 80%

respectively.       Conclusion       Ultrasound radiation triggers drugs releasing from MBs

which might be used as an effective drug carrier that combining ultrasound image and drug delivery in the field of oncology treatment.