嗅鞘细胞移植后通过分泌MMP-3在RCS-P<sup>+</sup>大鼠视网膜中迁移

谢晶; 李瑶琛; 阴正勤

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嗅鞘细胞移植后通过分泌MMP-3在RCS-P⁺大鼠视网膜中迁移

论著 | 更新时间：2026-01-09

- 嗅鞘细胞移植后通过分泌MMP-3在RCS-P⁺大鼠视网膜中迁移
- Transplanted olfactory ensheathing cells migrate in RCS-P⁺rat retina through secreting MMP-3
- 陆军军医大学学报 2012年34卷第9期页码：843-847
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  中图分类号： R322.91;R329.28;R339.12
- 纸质出版：2012
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谢晶,李瑶琛,阴正勤.嗅鞘细胞移植后通过分泌MMP-3在RCS-P+大鼠视网膜中迁移[J].陆军军医大学学报(原第三军医大学学报),2012,34(09):843-847.

　Xie Jing,Li Yaochen,Yin Zhengqin.Transplanted olfactory ensheathing cells migrate in RCS-P+ rat retina through secreting MMP-3[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(09):843-847.
谢晶,李瑶琛,阴正勤.嗅鞘细胞移植后通过分泌MMP-3在RCS-P+大鼠视网膜中迁移[J].陆军军医大学学报(原第三军医大学学报),2012,34(09):843-847. DOI：

　Xie Jing,Li Yaochen,Yin Zhengqin.Transplanted olfactory ensheathing cells migrate in RCS-P+ rat retina through secreting MMP-3[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(09):843-847. DOI：

摘要

目的初步研究将嗅鞘细胞（olfactory ensheathing cells，OECs）及嗅球成纤维细胞（olfactory nerve fibroblasts，ONF）混合细胞移植到皇家外科学院大鼠（Royal College of Surgeon rat，RCS-P

rat）的视网膜下腔后，OECs/ONF迁移进入视网膜的机制。方法离体实验中，取成年RCS-rdy

-P

大鼠的嗅球培养OECs/ONF至14 d行OECs/ONF的基质金属蛋白酶-3（matrix- metalloproteinase-3，MMP-3)细胞免疫荧光染色。收集5、8、11、14 d OECs/ONF培养液上清，与普通培养液超滤后进行酶联免疫吸附实验（ELISA），检测MMP-3的含量变化。在体实验中，制作40只RCS-P

大鼠单眼视网膜下腔细胞移植，并以对侧眼作为伪手术组以及相同天龄未处理大鼠作为对照组。术后7、14、21、28 d用ELISA法检测细胞移植组、伪手术组及未处理组大鼠视网膜中MMP-3含量的变化。将携带绿色荧光的慢病毒感染后的OECs/ONF移植到4只RCS-P

大鼠视网膜下腔，激光共聚焦显微镜观察移植后7、14、21 d及28 d OECs/ONF在视网膜中迁移情况。结果离体实验中，培养14 d时OECs/ONF 的MMP-3免疫细胞化学染色阳性。OECs/ONF培养液上清MMP-3含量分别为5 d （2.83±0.80）、8 d （6.34±1.12）、11 d （11.65±1.35）、14 d（19.11±2.11），明显高于普通D/F12+10%FBS培养液（1.65±0.44）（P0.01）；在体实验中，移植后21 d及28 d， OECs/ONF移植组视网膜MMP-3的含量[（1.80±0.29）、（3.96±0.51）

]

明显高于伪手术组[（1.17±0.20）、（1.83±0.26）

]

和未处理组[（1.19±0.17）、（1.92±0.25）

]

（P0.01）

伪手术组与未处理组之间无明显统计学差异（P0.05）。激光共聚焦显微镜观察可见移植后7～28 d，OECs/ONF在视网膜中迁移，最远能够达到神经节细胞层。结论 OECs/ONF移植到RCS视网膜下腔后，可能通过分泌MMP-3在RCS-P

大鼠视网膜中迁移。

Abstract

Objective To investigate how olfactory ensheathing cells (OECs) migrate in the retina of pigmented Royal College of Surgeon rats (RCS-P

rats) after being transplanted into the subretinal space with olfactory nerve fibroblasts (ONFs) together. Methods OECs/ONFs were primarily cultured from RCS-rdy

-P

rat olfactory bulbs and identified by immunofluorescence staining. The expression of anti-matrix metalloproteinase-3（MMP-3） in the OECs were detected by cytological immunological fluorescence staining. After GFP lentivirus infection

the secretion of MMP-3 in the supernatants of OECs collected at 5

and 14 d were detected by enzyme-linked immunosorbent assay (ELISA). For the experiment in vivo

the suspension of OECs/ONFs was injected into the subretinal space of one eye of 40 RCS-P

rats. PBS was injected into the subretinal space of the other eye. For the untreated group

no operation was done. In 7

21 and 28 d after surgery

their retina tissues were collected. ELISA was used to detect the changes of MMP-3 in the retina. After EGFP labeled OECs/ONF were injected into the subretinal space of 4 RCS-P

rats

laser confocal microscopy was used to observe the migration of OECs/ONF in 7

21 and 28 d after transplantation. Results In 14 d after culture

OECs/ONF secreted MMP-3. The MMP-3 level in the OECs/ONF supernatant was 2.83±0.80

6.34±1.12

11.65±1.35

19.11±2.11 at the 5th

8th

11th

and 14th days after culture respectively

which was significantly higher than that of control supernatants（1.65±0.44

P0.01）. For the experiment in vivo

the MMP-3 levels were 1.80±0.29 and 3.96±0.51 respectively in RCS-P

in 21 and 28 after OECs/ONF transplantation

which were significantly higher than those of the PBS group （1.17±0.20 and 1.83±0.26） and untreated group（1.19±0.17 and 1.92±0.25） (P0.01). There was no significant difference between the 2 latter groups (P0.05). From the 7th d to 28th d

laser confocal microscopy indicated that OECs/ONF migrated to the retina and most of them moved to the ganglion cell layer. Conclusion OECs/ONFs migrate into the retina of RCS-P

rats after being transplated into the subretinal space

which may be through secreting MMP-3.

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嗅鞘细胞移植后通过分泌MMP-3在RCS-P+大鼠视网膜中迁移

Transplanted olfactory ensheathing cells migrate in RCS-P+ rat retina through secreting MMP-3

DOI：

摘要

Abstract

关键词

Keywords

references

嗅鞘细胞移植后通过分泌MMP-3在RCS-P⁺大鼠视网膜中迁移

Transplanted olfactory ensheathing cells migrate in RCS-P⁺rat retina through secreting MMP-3