Objective        To investigate the effect of MS-275

a class Ⅰ histone deacetylase inhibitor

on the adipogenic differentiation of mesenchymal stem cells C3H/10T1/2.       Methods        MTT assay and FCM analysis was used to detect the effect of MS-275 on cell viability and cell cycle of C3H/10T1/2 cells. The effect of MS-275 on the adipogenic capacity of C3H/10T1/2 cells were dete

rmined by Oil red O staining. Real-time quantitative polymerase chain reaction (real-time PCR) was employed to detect the mRNA transcription levels of adipogenic differentiation markers

including fat binding protein (aP2)

perilipin

adiponectin (Adipoq) and adipogenic differentiation key transcription factor peroxisome proliferators-actiated receptor-gamma 2 (PPAR-γ2) after MS-275 treatment.       Results        MS-275 inhibited the viability of C3H/10T1/2 cells in a dose-dependent manner

and the IC

50

was identified nearly to 8 μmol/L (P0.05).FCM analysis showed that the cell cycle of C3H/10T1/2 was arrested at G

0

/G

1

phase after MS-275 treatment. Oil red O staining showed that 0.5 μmol/L MS-275 significantly reduced lipid drops accumulation in C3H/10T1/2 cells

and the mRNA transcription levels of aP2

perilipin

Adipoq and adipogenic differentiation key transcription factor PPAR-γ2 were decreased correspondingly (P0.05).       Conclusion        MS-275 attenuates the adipogenic differentiation capacity of mesenchymal stem cells

suggesting that inhibiting the activity of classⅠHDACs may partially suppress mesenchymal stem cells into adipogenesis.