Objective      To develop and validate a new approach for locating operation target of Parkinson’s disease (subthalamic nucleus

STN) based on the data set of Chinese visible human Ⅱ (CVH2).       Methods      Continuous transverse-section images of CVH2 head were chosen and a CVH2 stereotactic space was established. Transverse and coronal T2-weighted magnetic resonance images (MRI) of the brain (3.0 T) were obtained from 21 healthy adults. Then the anterior commissures (AC) and posterior commissures (PC) were used as intracranial localization markers to register all the MRI sequences into CVH2 stereotactic space. Accuracy of the registration was tested by comparing the centers of bilateral red nuclei (RN) in the CVH2 with those in the MRI.       Results      After registration

the mean deviations were 0.33 and 0.40 mm in X axis

0.52 and 0.44 mm in Y axis

and 0.64 and 0.64 mm in Z axis with standard deviations of 0.21 and 0.25 mm

0.39 and 0.36 mm

and 0.43 and 0.41 mm

respectively. There was no significant difference of RN coordinates between the registered MRI and CVH2 (P0.05).       Conclusion      The space registration method based on CVH2 provides an accurate means to locate RN which is adjacent to STN

and can be used for indirectly locating STN before deep brain stimulation for Parkinson’s disease treatment.