Objective       To explore the effect of Borna disease virus (BDV) infection on the proliferation and apoptosis in human oligodendrocytes (OL cells).       Methods       BDV infection of OL cells was detected by P40 immunofluorescence

cell growth was observed by CCK8 assay

and the apoptosis and cell cycle were detected by flow cytometry. Relative expression levels of Bax and Bcl-2 were also detected by Western blotting.       Results       Compare to negative control cells

the expression of P40 protein was positive in the experimental cells in 3 d a

fter BDV infection. As the infection time increased

the proliferation of OL cells were decreased. Flow cytometry indicated that BDV infection resulted in an increase of apoptotic cells in 3 d after infection. BDV infected cells were arrested at G

2

phase

and cell proliferation was inhibited. Expression of pro-apoptotic protein Bax was increased

and that of antiapoptotic protein Bcl-2 was decreased.       Conclusion       BDV infection inhibits cell proliferation

and induces cell apoptosis through up-regulating Bax expression and down-regulating Bcl-2 expression in OL cells.