内侧内嗅皮层微注射Orexin-A对大鼠睡眠-觉醒及空间记忆的影响

齐爱萍; 何超; 夏建霞; 张丹; 陈芳; 阎洁; 胡志安

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内侧内嗅皮层微注射Orexin-A对大鼠睡眠-觉醒及空间记忆的影响

论著 | 更新时间：2026-01-09

- 内侧内嗅皮层微注射Orexin-A对大鼠睡眠-觉醒及空间记忆的影响
- Effect of Orexin-A injected into medial entorhinal cortex of rats on sleep-wakefulness and spatial memory
- 陆军军医大学学报 2012年34卷第8期页码：715-718
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  中图分类号： R338.25;R338.6;R341
- 纸质出版：2012
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齐爱萍,何超,夏建霞,张丹,陈芳,阎洁,胡志安.内侧内嗅皮层微注射Orexin-A对大鼠睡眠-觉醒及空间记忆的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(08):715-718.

　Qi Aiping,He Chao,Xia Jianxia,Zhang Dan,Chen Fang,Yan Jie,Hu Zhian.Effect of Orexin-A injected into medial entorhinal cortex of rats on sleep-wakefulness and spatial memory[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(08):715-718.
齐爱萍,何超,夏建霞,张丹,陈芳,阎洁,胡志安.内侧内嗅皮层微注射Orexin-A对大鼠睡眠-觉醒及空间记忆的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(08):715-718. DOI：

　Qi Aiping,He Chao,Xia Jianxia,Zhang Dan,Chen Fang,Yan Jie,Hu Zhian.Effect of Orexin-A injected into medial entorhinal cortex of rats on sleep-wakefulness and spatial memory[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(08):715-718. DOI：

摘要

目的探讨内嗅皮层注射Orexin-A对Sprague-dawley（SD）大鼠睡眠-觉醒周期以及对空间记忆的影响。方法选用健康成年雄性SD大鼠45只，体质量250~300 g，采用同步记录大鼠脑电和肌电活动，观察Orexin-A及Orexin1受体阻断剂SB-334867内侧内嗅皮层微注射后大鼠睡眠-觉醒周期变化。采用T-迷宫交替选择次数，观察SB-334867内侧内嗅皮层微注射后大鼠T-迷宫选择正确率的变化。结果以人工脑脊液（ACSF）、二甲亚砜（DMSO）自身对照相比，分别给予大鼠内侧内嗅皮层双侧微注射Orexin A及SB-334867 0.5 μl后3 h（11：00-12：00、12：00-13：00、13：00-14：00）觉醒周期时间无影响（P0.05），给药后24 h觉醒周期时间也无显著差异（P0.05）。微量注射药物后，Orexin-A(0.1 nmol/0.5 μl)及SB-733467(6 mmol/0.5 μl)在11：00-14：00 3 h内对觉醒时间、NREM时间及REM时间均无影响（P0.05），即对3 h内（11：00-14：00）大鼠总睡眠觉醒时间无显著性差异（P0.05）。与DMSO组比较大鼠内侧内嗅皮层双侧微注射SB-334867 0.5 μl后，T-迷宫交替选择正确次数明显减少，正确率下降，有显著性差异（P0.001）。结论内侧内嗅皮层Orexin系统的支配可能参与了空间记忆，而不参与睡眠-觉醒周期调节。

Abstract

Objective To study the effect of Orexin-A injected into medial entorhinal cortex (MEC) of rats on sleep-wake cycle and spatial memory of SD rats. Methods Forty-five healthy adult male SD rats

weighing 250 to 300 g

were included in this study. Their cerebral and myoelectrical activities were synchronically recorded

their sleep-wake cycle and accuracy rate of alternative selection in T-maze were observed after injection of Orexin-A and SB-334867. Results Oexin-A and SB-334867 showed no significant effect on sleep-wake cycle

NREM and REM sleep time of rats 3 h after they were injected into MEC of rats compared with that of ACSF and DMSO（P0.05）. However

the accuracy rate of alternative selection in T-maze was significantly lower after injection of SB-733467 than after injection of DMSO（P0.001）. Conclusion The Orexin system in MEC may be involved in spatial memory but not in sleep-wake cycle.

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