L-4F对oxLDL刺激下脂肪细胞表达单核细胞趋化蛋白-1的影响

谢湘竹; 赵水平; 于碧莲; 钟巧青

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L-4F对oxLDL刺激下脂肪细胞表达单核细胞趋化蛋白-1的影响

论著 | 更新时间：2026-01-09

- L-4F对oxLDL刺激下脂肪细胞表达单核细胞趋化蛋白-1的影响
- Effect of L-4F on monocyte chemoattractant protein-1 expression in 3T3-L1 adipocytes induced by oxidized low-density lipoprotein
- 陆军军医大学学报 2012年34卷第10期页码：917-920
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  中图分类号： R329.2;R392-33;{R392.13}
- 纸质出版：2012
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谢湘竹,赵水平,于碧莲,钟巧青.L-4F对oxLDL刺激下脂肪细胞表达单核细胞趋化蛋白-1的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(10):917-920.

　Xie Xiangzhu,Zhao Shuiping,Yu Bilian,Zhong Qiaoqing.Effect of L-4F on monocyte chemoattractant protein-1 expression in 3T3-L1 adipocytes induced by oxidized low-density lipoprotein[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(10):917-920.
谢湘竹,赵水平,于碧莲,钟巧青.L-4F对oxLDL刺激下脂肪细胞表达单核细胞趋化蛋白-1的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(10):917-920. DOI：

　Xie Xiangzhu,Zhao Shuiping,Yu Bilian,Zhong Qiaoqing.Effect of L-4F on monocyte chemoattractant protein-1 expression in 3T3-L1 adipocytes induced by oxidized low-density lipoprotein[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(10):917-920. DOI：

摘要

目的观察载脂蛋白A-I模拟肽L-4F对氧化型低密度脂蛋白（oxLDL）刺激下3T3-L1脂肪细胞分泌表达单核细胞趋化蛋白-1（MCP-1）的影响，并探讨其可能的作用机制。方法 3T3-L1脂肪细胞促分化成熟后，oxLDL（50 μg/ml）刺激脂肪细胞，给予L-4F(1-50 μg/ml)，H-89（10 μmol/L）及H-89＋L-4F（50 μg/ml）干预，收集细胞，测定脂肪细胞MCP-1上清液中的浓度和mRNA表达水平，以及脂肪细胞核因子C/EBPα、β的蛋白质水平；改良Boyden小室法检测不同干预组上清液对人外周血单核细胞趋化活性的影响。结果 OxLDL（50 μg/ml）刺激使分化成熟的3T3-L1脂肪细胞表达及分泌MCP-1明显增加，并使得诱导的单核细胞移动距离明显增加。L-4F以浓度依赖的方式减少脂肪细胞MCP-1的表达和分泌，降低单核细胞趋化活性；50 μg/ml L-4F使MCP-1 mRNA的表达降低（91±6）％（P0.01）。PKA抑制剂H-89（10 μmol/L）干预oxLDL刺激的脂肪细胞后MCP-1 m RNA的表达也显著减少（P0.01），但是，在50 μg/ml L-4F 作用的基础上，H-89（10 μmol/L）的孵育并未使得MCP-1 mRNA的表达进一步降低（P0.05）。50 μg/ml oxLDL刺激对脂肪细胞C/EBPα的含量无明显影响，但增加C/EBPβ蛋白量，且该作用呈时间依赖性；L-4F和H-89干预均降低C/EBPβ的蛋白质含量。结论 OxLDL时间依赖性地诱导脂肪细胞C/EBPβ的蛋白合成，并增强脂肪细胞MCP-1的表达分泌，L-4F以浓度依赖的方式对抗oxLDL的致炎作用，cAMP/PKA-C/EBPβ信号通道可能是L-4F的作用途径之一。

Abstract

Objective To evaluate the effect of apolipoprotein A-I mimetic peptide L-4F on the secretion and expression of monocyte chemoattractant protein-1 (MCP-1) in 3T3-L1 adipocytes induced by oxidized low-density lipoprotein (oxLDL). Methods Fully differentiated 3T3-L1 adipocytes were preincubated in the medium containing different concentrations of L-4F (0

and 50 μg/ml)

H-89 (10 μmol/L) and H-89 (10 μmol/L) combined with L-4F (50 μg/ml)

respectively. Then the adipocytes were treated by oxLDL (50 μg/ml). The concentration of MCP-1

the mRNA expression of MCP-1

and the protein levels of CCAAT/enhancer binding protein (C/EBP)α and C/EBPβ in cell supernatant were evaluated. The effect of the supernatant on the chemotactic activity of human peripheral blood monocytes was detected by micropore filter method using a modified Boyden chamber. Results OxLDL (50 μg/ml) treatment induced a significant increase of MCP-1 expression and secretion in 3T3-L1 adipocytes

and the mean of migration distance of human peripheral blood monocytes induced by the cell supernatant was significantly longer than that of the random migration group. MCP-1 secretion and expression as well as the chemotactic activity of human peripheral blood monocyte were decreased by the L-4F pre-incubation in a dose-dependent manner. MCP-1 expression in the adipocytes pretreated with L-4F (50 μg/ml) decreased by (91±6)% (P0.01). PKA inhibitor H-89 (10 μmol/L) significantly reduced the oxLDL-induced MCP-1 expression (P0.01)

but no further decrease was observed when H-89 (10 μmol/L) was used in combination with L-4F (50 μg/ml) (P0.05). OxLDL (50 μg/ml) treatment showed no significant effect on C/EBPα content

but increased C/EBPβ content in a time-dependent manner. H-89 and L-4F both attenuated C/EBPβ protein content in oxLDL-treated 3T3-L1 adipocytes. Conclusion OxLDL induces C/EBPβ protein synthesis and enhances MCP-1 secretion and expression in 3T3-L1 adipocytes in a time-dependent manner. L-4F dose-dependently counterbalances the proinflammatory effect of oxLDL

which may involve cAMP/PKA-C/EBPβ signaling pathway.

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