Objective      To investigate that whether hepatocyte cell adhesion molecule (hepaCAM) is actively secreted from renal cell carcinoma 786-0 cells by a pathway involving exosomes and whether hepaCAM could inhibit proliferation in 786-0 cells.       Methods      A recombinant adenovirus vector plasmid containing hepaCAM was transfected into 786-0 cells. The expression of hepaCAM was detected by Western blotting. The transfected cells were treated with monensin and 5-(N-N-Dimetyl)-amiloride hydrochloride (DMA)

and then extracellular culture medium and exosomes were used to investigate the content of hepaCAM by Bradford protein assay. MTT assay was used to detect the cell viability after treatment of exosomes cotaining hepaCAM.       Results      HepaCAM adenovirus vector plasmid was successfully constructed and stably transfected into 786-0 cells. Monensin enhanced the release of hepaCAM while DMA inhibited the process in the transfected 786-0 cells. Exosomes purityed from the supernatant of transfected 786-0 cells inhibited the proliferation of 786-0 cells obvously.       Conclusion      HepaCAM is secreted from renal cell carcinoma cells by a pathway involving exosomes

and inhibits cell proliferation at the same time.