Objective     To determine the relationship of the expression of Survivin and nm23-H1 protein with lymph node metastasis (LNM) in human colorectal carcinoma and cancer adjacent tissues.      Methods     The expression of Survivin and nm23-H1 protein was examined in 66 tissue samples of colorectal cancer and 15 cases of adjacent tissues using immunohistochemical S-P method

and the results were analyzed for its relationship with clinicopathological features.      Results     In colorectal carcinoma and adjacent tissues

Survivin positive rates were 75.7% and 62.1%

respectively

with significant difference (Chi sqaure=13.255

P=0.000);while those of nm23-H1 were 26.7% and 93.3% respectively

with statistically significance (Chi sqaure=4.125

P=0.042). Survivin protein expression was related with tumor differentiation

LNM

liver metastasis

Dukes stage (P0.05). The expression of nm23-H1 protein was related with LNM

liver metastasis

Dukes stage (P0.01). Positiveness to Survivin protein had positive correlation with the LNM (r=0.247

P=0.046)

while that to nm23-H1 protein had negative correlation with the LNM (r=-0.359

P=0.003). Negative correlation was found between Survivin and nm23-H1 expression (r=-0.296

P=0.016).      Conclusion     The anti-apoptotic effect of Survivin protein and the anti-metastastic effect of nm23-H1 protein may play important role in the occurrence and development of colorectal carcinoma. It may be an important prognostic indicator to determine lymph node metastatic potential and prognosis in human colorectal carcinoma using joint detection of them.