Objective      To determine the effect of histatin1 (Hst1) and recombinant human epidermal growth factor (rhEGF) on the proliferation and migration in human skin fibroblasts.        Methods      Human skin fibroblasts was subcultured

and then randomly divided into control group (DMEM+1%NBS)

A group (100 μg/ml Hst1)

B group (30 μg/ml Hst1) and C group (3 μg/ml Hst1). Cell proliferation was observed by growth curve at 24

48 and 72 h after corresponding treatment. Human skin fibroblasts was also randomly divided into control group (DMEM+1%NBS)

Ⅰgroup (30 μg/ml Hst1)

Ⅱgroup (10 ng/ml rhEGF)

Ⅲgroup (30 μg/ml Hst1+10 ng/ml rhEGF)

Ⅳgroup (15 μg/ml Hst1+5 ng/ml rhEGF)

and Ⅴgroup (15 μg/ml Hst1+10 ng/ml rhEGF). The ability of cell migration was evaluated by cell scrath test.       Results      ① The cell proliferation was significantly enhanced in B group and A group at 24 h（P0.01）.②Hst1 at 30 μg/ml resulted in remarkable increased cells in the healing area

with a healing rate of 37.7% at 8 h. The healing area rate in combination groups (Hst1+rhEGF) was significantly higher than that in 10 ng/ml rhEGF group or 30 μg/ml Hst1 group. After Mitomycin C treatment

which restrained the cells proliferation

the cells healing area was more elevated in 10 ng/ml rhEGF group

15 μg/ml Hst1 + 5 ng/ml rhEGF group and 15 μg/ml Hst1 +10 ng/ml rhEGF group

but not in 30 μg/ml Hst1 group when compared with that in control group. The healing area was declined at 8 h than that of Mitomycin C untreated

but the difference was no significant. The healing area in combination groups (Hst1+rhEGF) had no significantly difference with that in 10 ng/ml rhEGF group or 30 μg/ml Hst1 group.       Conclusion      Hst1 can induce the proliferation of human skin fibroblasts

but exert not significant effect on migration. rhEGF combined with Hst1 shows a synergic effect on wound healing.