Objective       To observe the effect of different glucose concentrations on expression of toll-like receptor-2 (TLR-2) in ARPE-19 cells.       Methods        ARPE-19 cells were divided into 5.5 mmol/L glucose group

15.5 mmol/L glucose group

25.5 mmol/L glucose

25.5 mmol/L glucose +10 mmol/L Go6976 PKC-α inhibition group

5.5 mmol/L glucose + 20 mmol/L mannitol control group. Expression level of TLR-2 in ARPE-19 cells and translocation of PKC-α from cytoplasm to cytomembrane were measured by Western blotting

while that of TLR-2 mRNA in ARPE-19 cells was measured by real-time PCR.       Results        The expression level of TLR-2 mRNA and the translocation of PKC-α from cytoplasm to cytomembrane were higher in 25.5 mmol/L glucose group and 15.5 mmol/L glucose group than in control group (P0.05). The expression level of TLR-2 protein and the translocation of PKC-α from cytoplasm to cytomembrane were significantly lower in PKC-α inhibitor inhalation group than in 25.5 mmol/L glucose group (P0.01).       Conclusion        High glucose concentration can up-regulate the expression of TLR2 mRNA in ARPE-19 cells and promote the translocation of PKC-α from cytoplasm to cytomembrane through the PKC-α pathway.