Objective     To study the effect of a mTOR specific inhibitor

everolimus (RAD001)

on cell cycle of gastric carcinoma cell line SGC7901 and the synergic inhibitory effect of combined RAD001 and cisplatin on proliferation of SGC7901 cells.      Methods     After SGC7901 cells were cultured in vitro and stained with HE

their morphology was observed after treated with RAD001 alone or in combination with cisplatin

and their cell cycle was assayed by flow cytometry. Expression of P21 in SGC7901 cells was detected by S-P immunecellchemistry and Western blotting

respec

tively.      Results     RAD001 could inhibit the proliferation of SGC7901 cells in a concentration-dependent manner. Combined RAD001 and cisplatin showed a synergic inhibitory effect on the proliferation of SGC7901 cells. The proportion of SGC7901 cells at G

0

-G

1

was increased and significantly higher in combined RAD001 and cisplatin treatment group than in single RAD001 treatment group (P0.05). The expression level of P21 in SGC7901 cells was significantly higher 48 h after combined RAD001 and cisplatin treatment than after single RAD001 treatment (P0.05).      Conclusion     RAD001 can inhibit the proliferation of SGC7901 cells by up-regulating the expressions of P21 and blocking the cell cycle of SGC7901 cells. Combined RAD001 and cisplatin has a synergic inhibitory effect on the proliferation of SGC7901 cells.