Objective     To study the effect of specific integrin-linked kinase (ILK) siRNA on expression of p-Akt and p-GSK3β and growth of transplanted bladder cancer tumor xenografts.      Methods     Specific ILK siRNA was transfected into human bladder cancer BIU-87 cells. BIU-87 cells stably expressing ILK siRNA were selected and subcutaneously injected into nude mice. Size and weight of the transplanted bladder tumor in nude mice were measured. Morphology of tumor tissue was observed with HE staining. Change of micro-blood vessels in tumor tissue and expression of p-Akt and p-GSK3β were detected by immunohistochemistry.      Results     The weight of tumor was significantly lighter in mice with BIU-87 siRNA than in those with normal and negative control BIU-87 cells (P0.01) with a tumor inhibitory rate of 64.73% and 66.73%

respectively. The volume of cancer cells

the ratio of nuclear plasma

the staining of nuclei

and karyokinesis were significantly less in mice with BIU-87 siRNA than in those with normal and negative control BIU-87 cells. Less micro-blood vessels were observed in mice with BIU-87 siRNA than in those with normal and negative control BIU-87 cells or no blood vessels were found in mice with BIU-87 siRNA. The p-Akt and p-GSK3β were weakly expressed in mice with BIU-87 siRNA.      Conclusion     Specific ILK siRNA can inhibit the growth of transplanted bladder cancer in nude mice by decreasing the expression of p-Akt and p-GSK3β.