Objective        To determine  the expression of acetylated histone in transcription regulation area of fatty acid synthase (FAS) gene with liver X receptor (LXR) agonist T0901317 and whether acetylated histone induces chromatin remodeling during expression of FAS gene transcription.       Methods        Transcription expression time spectrum of FAS mRNA was detected by real-time fluorescence quantitative PCR. H3 and H4 expression levels at 4 transcription regulation area sites of FAS gene were measured by chromatin immunoprecipitation and real-time fluorescence quantitative PCR respectively.       Results        The expression level of FAS mRNA increased in a time-dependent manner in 2 h after T0901317 was used (1.09

P0.05) and reached its peak at 16 h (4.25

P0.01)

which was significantly higher at 48 h than at 0 h (P0.01). The expression level of acetylated H3 was significantly higher at 30 min than at 0 h after T0901317 was used (P0.05)

and was lower than that at 0 h with a difference at 2 000 bp (P0.05) and close to or higher at 2 h than at 0 h with a difference at LXRE (P0.05). The expression level of acetylated H4 was also higher at 30 min than at 0 h after T0901317 was used (P0.05) and lower at 1 and 2 h than at 0 h (P0.05).       Conclusion        The expression level of FAS mRNA is increased in a time-dependent manner when the T0901317 is used. Acetylated histone at 2 500 bp upstream and downstream at the transcription start site during early FAS transcription induces chromatin remodeling with the site of transcription regulation area

the time when the T0901317 is used

and the expression level of histone.