Objective       To screen out miRNAs that regulate HBsAg expression

HBeAg expression and proliferation of HBV in HepG2 2.2.15 cells

and to provide evidences for interaction between host cell’s miRNAs and HBV.       Methods       Three hundred and twenty eight antisense oligonucleotides of miRNAs were used to inhibit expressions of endogenous miRNAs. Enzyme linked immunosorbent assay (ELISA) and real-time PCR were performed on culture supernatant

which was harvested from HepG2 2.2.15 cells at 72 h  after transfection with miRNA ASOs

to detect expressions of HBsAg and HBeAg and copy number of HBV DNA

respectively. Cell proliferation assay (MTS) was used at the same time.       Results        Three miRNAs

including miR-199a-3pASO

miR-210ASO and miR-185ASO

promoted HBsAg expression and HBV proliferation (P0.05). Overexpression of miR-199a-3p

miR-210 and miR-185 inhibited HBsAg expression and HBV proliferation (P0.05). Compared with those of the control group

HepG2 2.2.15 cells transfected with miR-199a-3pASO

miR-210ASO and miR-185ASO showed a decreased level of miR-199a-3p

miR-210 and miR-185

respectively (P0.05). Cells transfected with miR-199a-3p

miR-210 and miR-185 showed an increased levels of miR-199a-3p

miR-210 and miR-185 compared with those of the control group (P0.05).       Conclusion        miR-199a-3p

miR-210 and miR-185 inhibit HBsAg expression and HBV proliferation without inhibition of cell proliferation. miRNA

which regulates the interaction between host cell’s miRNAs and HBV replication

plays an important role in HBV proliferation.