Objective      To identify the biological activity of recombinant endolysin encoded by bacteriophage MmP1 against Morganella morganii.       Methods        The peptidoglycan of Morganella morganii was extracted. Enzymatic activity of the recombinant endolysin was detected by zymography assay and gel diffusion assay

respectively. The inhibitory activity of recombinant protein against S.aureus culture was detected by paper disk method. The synergistic effect of recombinant protein and antibiotics was determined by MIC.       Results      Zymography assay and gel diffusion assay showed that Morganella morganii peptidoglycan can be hydrolyzed by the recombinant endolysin. MmP1 endolysin could inhibit growth of S.aureus. The synergistic effect was observed when MmP1 endolysin was combined with antibiotics including AmpⅡ

Amp and Car

and the MIC of AmpⅡ

Amp and Car reduced from 0.39 μg/ml

0.78 μg/ml and 0.195 μg/ml to 1.56 μg/ml

1.56 μg/ml and 3.125 μg/ml

respectively.       Conclusion      The recombinant endolysin can hydrolyze Morganella morganii peptidoglycan

inhibit growth of S.aureus

and enhance bacteriostasis activity of antibiotics.