三羟基异黄酮联合放疗对人肺腺癌细胞增殖凋亡的影响

周芊; 李少林; 杨镇洲; 王东; 李梦侠; 曾林立

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三羟基异黄酮联合放疗对人肺腺癌细胞增殖凋亡的影响

论著 | 更新时间：2026-01-09

- 三羟基异黄酮联合放疗对人肺腺癌细胞增殖凋亡的影响
- Effect of Genistein combined with radiotherapy on cell proliferation and apoptosis in pulmonary adenocarci noma A549 cells
- 陆军军医大学学报 2011年33卷第19期页码：2008-2011
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  中图分类号： R73-36;R730.23;R734.2
- 纸质出版：2011
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周芊,李少林,杨镇洲,王东,李梦侠,曾林立.三羟基异黄酮联合放疗对人肺腺癌细胞增殖凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(19):2008-2011.

　Zhou Qian,Li Shaolin,Yang Zhenzhou,Wang Dong,Li Mengxia,Zeng Linli.Effect of Genistein combined with radiotherapy on cell proliferation and apoptosis in pulmonary adenocarci noma A549 cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(19):2008-2011.
周芊,李少林,杨镇洲,王东,李梦侠,曾林立.三羟基异黄酮联合放疗对人肺腺癌细胞增殖凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(19):2008-2011. DOI：

　Zhou Qian,Li Shaolin,Yang Zhenzhou,Wang Dong,Li Mengxia,Zeng Linli.Effect of Genistein combined with radiotherapy on cell proliferation and apoptosis in pulmonary adenocarci noma A549 cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(19):2008-2011. DOI：

摘要

目的观察三羟基异黄酮（Genistein）联合放疗对人肺腺癌细胞A549增殖凋亡及其Ape1/Ref1蛋白的影响。方法 1组单用0、10、30、60、90 μmol/L的Genistein处理A549细胞48 h，另1组用同样浓度的Genistein预处理24 h后予以直线加速器放疗8 Gy，继续培养24 h后用噻唑蓝比色法(MTT)检测各组活细胞数，确定最适Genistein联合放疗的浓度。用流式细胞仪检测对照组、单纯放疗组（IR 8 Gy）、单纯Genistein组（GEN 30 μmol/L）、Genistein+IR组(GEN 30 μmol/L+IR 8 Gy)的细胞凋亡；采用Western blot法检测各组细胞中Ape1/Ref1蛋白表达。结果 30、60、90 μmol/L的Genistein联合放疗比单用相应浓度的Genistein处理细胞增殖抑制率高(P0.05)，但低浓度10 μmol/L联合放疗与单纯放疗比较差异不明显[（26.67±1.26）% vs （24.70±0.72）%，P=0.102]。IR组、Genistein+IR组早期凋亡率较对照组明显增高[（30.88±7.63）%、（42.98±1.46）% vs （6.80±0.98）%，P0.05]，加药组较对照组无明显统计学差异[（12.96±4.99）% vs （6.80±0.98）%，P=0.143)]；与对照组、单纯放疗组、单纯加药组比，Genistein+IR组中Ape1/Ref1的表达明显下调。结论三羟基异黄酮可增加放疗对A549细胞的增殖抑制作用，可能与减少Ape1/Ref1蛋白的表达有关。

Abstract

Objective To examine the effect of Genistein combined with radiotherapy on the proliferation

apoptosis and Ape1/Ref1 protein expression of human pulmonary adenocarcinoma cell line A549. Methods A549 cells were divided into 4 groups including a control group (cells with no treatment)

a Genistein group (cells treated with Genistein in the doses of 0 μmol/L

10 μmol/L

30 μmol/L

60 μmol/L and 90 μmol/L

respectively

for 48 h)

an IR group (cells treated with a 8 Gy irradiation) and a Genistein +IR group (cells pretreated with the same doses of Genistein for 24 h and with a 8 Gy irradiation by a linear accelerator and continuously cultured for another 24 h). The cell proliferation was examined by methyl thiazolyl tetrazolium (MTT) assay to determine a most suitable Genistein concentration combined with radiotherapy. Cell apoptosis of the 4 groups was observed by flow cytometry

and Ape1/Ref1 protein expression was measured by Western blotting. Results The inhibition rate of cell proliferation was higher in the treatment of 30 μmol/L，60 μmol/L and 90 μmol/L Genistein combined with irradiation than in the same doses of Genistein treatment alone (P0.05)

and the most suitable concentration of Genistein was 30 μmol/L. No significant difference was found between the cells treated with low concentration (10 μmol/L) of Genistein combined with irradiation and with irradiation alone [(26.67±1.26)% vs (24.70±0.72)%

P=0.102]. The early apoptotic rates of the IR group and the Genistein+IR group were significantly higher than that of the control group[(30.88±7.63)%

(42.98±1.46)% vs (6.80±0.98)%

respectively

P0.05]

while there was no statistical difference between the control group and the Genistein group [(12.96±4.99)% vs (6.80±0.98)%

P=0.143]. As compared with the Ape1/Ref1 protein expression of the control group

the IR group and the Genistein group

that of the Genistein+IR group decreased significantly. Conclusion Genistein (30 μmol/L) can enhance the inhibition effect of radiotherapy on A549 cell proliferation through the reduction of Ape1/Ref1 protein expression.

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