儿童结核病101例临床分离结核分枝杆菌多位点串联重复序列分型

王均; 黄延风; 张爱华; 许红梅

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儿童结核病101例临床分离结核分枝杆菌多位点串联重复序列分型

论著 | 更新时间：2026-01-09

- 儿童结核病101例临床分离结核分枝杆菌多位点串联重复序列分型
- Genotyping of 101 Mycobacterium tuberculosis isolates from pediatrics tuberculosis in Chongqing by multiple locus VNTR analysis
- 陆军军医大学学报 2011年33卷第13期页码：1408-1410
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  中图分类号： R372;R378.911;R394.3
- 纸质出版：2011
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王均,黄延风,张爱华,许红梅.儿童结核病101例临床分离结核分枝杆菌多位点串联重复序列分型[J].陆军军医大学学报(原第三军医大学学报),2011,33(13):1408-1410.

　Wang Jun,Huang Yanfeng,Zhang Aihua,Xu Hongmei.Genotyping of 101 Mycobacterium tuberculosis isolates from pediatrics tuberculosis in Chongqing by multiple locus VNTR analysis[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(13):1408-1410.
王均,黄延风,张爱华,许红梅.儿童结核病101例临床分离结核分枝杆菌多位点串联重复序列分型[J].陆军军医大学学报(原第三军医大学学报),2011,33(13):1408-1410. DOI：

　Wang Jun,Huang Yanfeng,Zhang Aihua,Xu Hongmei.Genotyping of 101 Mycobacterium tuberculosis isolates from pediatrics tuberculosis in Chongqing by multiple locus VNTR analysis[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(13):1408-1410. DOI：

摘要

目的了解重庆地区儿童结核病中结核分枝杆菌临床分离株的数目可变串联重复序列（variable number tandem repeats

VNTR）的分布特征，寻找适合的VNTR位点组合。方法采用多位点串联重复序列（multiple locus VNTR analysis

MLVA）分型方法，选择标化的24个VNTR位点，对101例结核分枝杆菌临床分离株DNA进行检测，结果采用BioNumerics 6.1数据库软件进行聚类分析和单位点Hunter-Gaston分辨率指数（Hunter-Gaston index

HGI）分析，并比较分析国际推荐的分组（12、15、24位点）的基因分型鉴定能力。结果 MLVA分析结果显示24个VNTR位点在不同菌株中存在明显的多态性，101例结核分枝杆菌临床分离株可分为1个基因群83种基因型，其中69种基因型只有1个菌株，占68.32%（69/101），另有32株临床分离株表现出14种基因型，占31.68%（32/101），成簇率为17.82%； 24个VNTR位点的HGI具有较大差异（0.168~0.829），HGI能达到0.5以上的VNTR位点数有16个；24个VNTR位点进行不同的位点组合：12、15个和24个位点组合的HGI分别为0.995、0.996、0.996。结论重庆地区儿童结核病中结核分枝杆菌存在基因多态性，国际推荐的15个VNTR位点组合的MLVA分型方法适用于其流行病学的研究。

Abstract

Objective To study the distribution of the variable number tandem repeats (VNTR) in clinically isolated strains Mycobacterium tuberculosis (M. tuberculosis) from pediatrics tuberculosis in Chongqing and explore the effective loci combination of them by multiple locus VNTR analysis (MLVA). Methods Twenty-four standardized MLVA loci was used to type the 101 clinically isolated M. tuberculosis strains

and the results were analyzed by BioNumerics 6.1 software

which including dendrogram and Hunter-Gaston index (HGI) of 24 loci. The HGI of different loci set (12 loci

15 loci

and 24 loci) was evaluated. Results Twenty-four loci showed different polymorphism. All the 101 clinical isolates were typed into one group that displaying 83 genotypes

in which 69 strains (68.32%

69/101) were typed 69 genotypes respectively

and 32 strains (31.68%) were typed 14 genotypes which showed cluster rate of 17.82%. The HGI of 24 loci was from 0.168 to 0.829

the number of VNTR loci with HGI higher than 0.5 was 16. Three loci set displayed different polymorphism. HGI of 12 loci

15 loci

and 24 loci set were 0.995

0.996

and 0.996 respectively. Conclusion The clinical strains of M. tuberculosis isolated from pediatrics tuberculosis in Chongqing show definite polymorphism and the standardization 15 loci VNTR set of MLVA can be used in the molecular epidemiological study of these strains in Chongqing.

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