Objective      To investigate the influence of Has-miR-193b on the invasion of human endometrium adenocarcinoma HEC-1B cells.      Methods      Validated Has-miR-193b mimics and inhibitors were transfected into endometrial carcinoma HEC-1B cells. Real-time PCR was used to confirm the efficiency of transfection. Invasiveness of transfected and non-transfected HEC-1B cells was detected by matrix metrigel invasion assay. miRGen

Targetscan

and Pictar database was used to find target genes of Has-miR-193b. Western blotting was used to measure GRB7 protein expression in the cells.      Results      Hsa-miR-193b expression in HEC-1B cells was significantly change after mimics and inhibitor transfection (P0.05). Has-miR-193b mimics transfection resulted in decrease of invasiveness in HEC-1B cells (P0.05)

and the decrease of GRB7 protein expression (P0.05). While the expression was increased after transfected with Has-miR-193b inhibitor. But no obvious change was found in the GRB7 mRNA expression in nontransfected

mimics-transfected and inhibitor-transfected HEC-1B cells.      Conclusion      Transfection with Has-miR-193b mimics effectively down-regulates GRB7 expression

and suppresses invasiveness in HEC-1B cells.