Objective     To screen the differential expression microRNAs in human dental pulp cells under the undifferentiation condition and in the mineralization induction fluid

and forecast target genes.     Methods  After human dental pulp cells were isolated from outpatients and then identified

these cells were separately cultured in the ordinary culture medium or the mineralization induction culture medium. The formation of cellular nodules were observed

and the cell differentiation was observed by Vonkossa staining. The differential expression of microRNA was detected by microRNA chip

and target genes were forecast by miRGen database.Functional rich products of these target genes were assayed by GO and KEGG Pathway.     Results      Compared with the human dental pulp cells cultured in the ordinary culture medium

there were 72 microRNAs expressing over 1.5-fold up-regulated and 61 microRNAs expressing over 1.5-fold down-regulated in the mineralization induction culture medium. There were 35 miRNAs with target genes by microRNA target forecast tool analysis. The total target genes of 35 miRNAs was 1 327

and the total relations of miRNA and target gene were 2 158.     Conclusion      We screen miRNAs signature of human dental pulp cells and forecast some target genes.