Objective      To study the expression of survivin gene in adult stem cells and the activities of 3 cloned survivin gene promoter segments with different lengths.     Methods     Fibroblasts (10T

1/2

)

myoblasts (C

2

C

12

)

dermal multifunctional cells (DMSC)

human bone mesenchymal cells (BMSC)

colonic adenoma cells (HT

29

)

and human nephroblasts (293FT) were cultured. Expressions of survivin gene in adult stem cells and tumor cells were det

ected by RT-PCR with immunochemical staining. Survivin gene promoter vector with different lengths was constructed with luciferase and transfected into 293FT with lipid some. Activity of luciferase reflecting the activity of promotors was assayed.     Results      The survivin gene was mildly expressed in normal adult stem cells

moderately expressed in fibroblasts

and well expressed in colonic adenoma cells (HT

29

). Survivin gene promoters with different lengths were cloned and linked to the upstream of luciferase cDNA. The activity of 987 bp- and 160 bp-long survivin gene promoters was higher than that of 270 bp-long survivin gene promoter in 293FT.     Conclusion     The survivin gene promoter can be used in monitoring and treatment of the malignant transformation of implanted stem cells by constructing its expression vector because of the tumor-specific and general expression characteristics. The vector constructed with highly active survivin gene promoter can improve the efficacy of gene therapy.