Objective    To explore the influence of X-box binding protein 1 (XBP1) on the expression of inflammatory factor of lipopolysaccharide (LPS)-induced Kupffer cells (KCs).     Methods    KCs derived from BALB/c mice were randomly divided into an XBP1-shRNA group

a negative control group and a blank control group

which received XBP1-shRNA plasmid transfection

control vector transfection and no treatment

respectively. Then the three groups were all treated with LPS. The mRNA and protein expression of XBP1 in KCs was determined by Real-time PCR

Western blotting and immunohistochemistry. The activity of NF-κB and the density of TNF-α in the supernatant of coculture were assessed by enzyme-linked immunosorbent assay (ELISA).     Results    The expression of XBP1 and its downstream factor TNF-α was significantly lower than that in the negative control group and blank control group (P0.05). However

there was no significant difference between the two control groups (P0.05). In addition

the activity of NF-κB in the three groups had no significant difference (P0.05).     Conclusion    XBP1-shRNA plasmid can significantly inhibit the expression of XBP1 gene and reduce the release of its downstream factor TNF-α without changing the activity of NF-κB in KCs.