Objective      To explore the effect of self-made liver solution on hepatocyte injury and MRPK signal transcriptional pathway after portal vein occlusion in rats with obstructive jaundice.      Methods      Seventy-two Wistar rats were randomly divided into four groups

group A (biliary tract recanalization

liberation of portal vein

resection of caudate lobe of liver and closure of abdomen in 90 min)

group B (biliary tract recanalization

portal vein bypass in caudate lobe of liver

PVO of left hepatic tissue in 90 min

then recovery of portal vein flow and resection of caudate lobe of liver)

group C (group B with perfusion of 4 ℃ Lactated Ringer’s Solution) and group D (group B with portal vein perfusion of 4 ℃ self-made liver solution). Liver samples were taken at 0

6 and 24 h post operation. Reverse transcription PCR

Western blotting and TUNEL were used respectively to determine A20 mRNA

A20 protein

p-JNK

p-ERK

p-p38

Bcl-2

Bax

Caspase-3 and hepatocyte apoptotic rate.      Results      The levels of A20 mRNA and protein were increased obviously in group D compared to the other groups (P0.05). The expression of p-JNK

Bax

and Caspase-3 was decreased but the expression of p-ERK and Bcl-2 protein was presented the adverse changes. The hepatocyte apoptotic rates of group C and group D were obvious lower than that of group B.      Conclusion      Continuous hypothermical perfusion with self-made liver solution improves expression of anti-apoptosis protein A20

then activates ERK signal transduction pathway to regulate the balance of Bcl-2/Bax protein and decreases the expression of apoptosis executive protein Caspase-3.