实时定量PCR检测miR-373*、miR-200c在乳腺癌干细胞中的表达及其靶基因预测

邱钧; 金俊余; 孙建国; 廖荣霞; 王欣欣; 陈正堂

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实时定量PCR检测miR-373*、miR-200c在乳腺癌干细胞中的表达及其靶基因预测

论著 | 更新时间：2026-01-09

- 实时定量PCR检测miR-373*、miR-200c在乳腺癌干细胞中的表达及其靶基因预测
- Detection of miR-373* and miR-200c expression in breast cancer stem cells by real-time PCR and prediction of their target genes
- 陆军军医大学学报 2011年33卷第10期页码：984-987
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  中图分类号： R394.3;R730.3;R737.9
- 纸质出版：2011
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邱钧,金俊余,孙建国,廖荣霞,王欣欣,陈正堂.实时定量PCR检测miR-373*、miR-200c在乳腺癌干细胞中的表达及其靶基因预测[J].陆军军医大学学报(原第三军医大学学报),2011,33(10):984-987.

　Qiu Jun,Jin Junyu,Sun Jianguo,Liao Rongxia,Wang Xinxin,Chen Zhengtang.Detection of miR-373* and miR-200c expression in breast cancer stem cells by real-time PCR and prediction of their target genes[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(10):984-987.
邱钧,金俊余,孙建国,廖荣霞,王欣欣,陈正堂.实时定量PCR检测miR-373*、miR-200c在乳腺癌干细胞中的表达及其靶基因预测[J].陆军军医大学学报(原第三军医大学学报),2011,33(10):984-987. DOI：

　Qiu Jun,Jin Junyu,Sun Jianguo,Liao Rongxia,Wang Xinxin,Chen Zhengtang.Detection of miR-373* and miR-200c expression in breast cancer stem cells by real-time PCR and prediction of their target genes[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(10):984-987. DOI：

摘要

目的分析miR-373*、miR-200c在乳腺癌干细胞(breast cancer stem cells，BCSCs)和MCF-7乳腺癌细胞株中的差异表达。方法 Trizol法分别抽提乳腺癌干细胞和MCF-7细胞总RNA，实时定量PCR检测miRNAs 表达，对表达差异显著的miRNAs进行潜在靶基因预测。结果 miR-373*在乳腺癌干细胞中较MCF-7细胞高表达（6.684±0.548）

而miR-200c在乳腺癌干细胞中较MCF-7细胞低表达(0.345±0.031）。通过软件分析预测，miR-373*与TP53INP2、CASP8、EIF4A1、EEF1A1等基因均具有可能靶位点，miR-200c与TCF2、TDE2、SYVN1、PDCD10、RAP2C等基因均具有可能靶位点。结论 miR-373*在乳腺癌干细胞中高表达，可能是一个潜在的癌基因。miR-200c在乳腺癌干细胞中低表达，可能是一个潜在的抑癌基因。

Abstract

Objective To analyze the differential expression of miR-373* and miR-200c in breast cancer stem cells (BCSCs) and breast cancer cell line MCF-7. Methods Total RNA of BCSCs and MCF-7 cells were extracted with Trizol. MicroRNA (miRNA) expression was detected by real-time PCR. The potential target genes of miRNAs that exhibited significant differential expression were predicted using software PicTar

miRanda

and Targetscan. Results Compared with those in MCF-7 cells

the expression levels of miR-373* and miR-200c were about 7 times higher (fold change=6.684±0.548) and about 3 times lower (fold change=0.345±0.031)

respectively

in BCSCs. Prediction software analysis indicated that miR-373* had potential target sites in TP53INP2

CASP8

EIF4A1 and EEF1A1

and miR-200c had potential target sites in TCF2

TDE2

SYVN1

and some RAS oncogene family members. Conclusion In comparison with MCF-7 cells

BCSCs show up-regulated expression of miR-373*

which may be a potential oncogene

and down-regulated expression of miR-200c

which may be a potential anti-oncogene.

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