Objective    To analyze the proteins of immature mouse bone marrow-derived dendritic cells (BMDCs) by 2-dimentional electrophoresis (2-DE) and peptide mass fingerprinting.     Methods    Interleukin-4 (IL-4) and granulocyte-macrophage colony-stimulating factor (GM-CSF) were employed to induce differentiation of mouse bone marrow cells into immature mouse BMDCs. Proteins were extracted from the DCs

separated by 2-DE

stained by improved silver method

analyzed by PDQuest software

and identified by peptide mass fingerprinting and MS-Fit.     Results    High-purity immature mouse BMDCs were obtained

as confirmed by detecting cell growth condition and surface mark

ers. Image analysis result showed that  660±10 protein spots were separated and mainly distributed in a relative molecular weight range of (28-100)×10

3

and an isoelectric point range of 5.0-9.0. Among the 87 identified spots

40 (45.98%) were related to immunoregulation function of immature DCs and 37 (42.53%) to macromolecular metabolism.     Conclusion    Six hundred and sixty proteins are separated from immature mouse BMDCs

and 87 proteins are identified.