Objective      To investigate the genotype distribution of TRB3 exon+251A/G in Han population from Chongqing area

and to explore its correlation with polycystic ovarian syndrome (PCOS)

glycolipids metabolism and insulin resistance.      Methods      A total of 139 PCOS patients and 120 matched normal subjects were genotyped by PCR-restriction fragment length polymorphism (PCR-RFLP). Waist circumference

hip circumference

body mass index

plasma glucose

serum insulin

lipid profiles and total testosterone were measured. In a subgroup of this study

the hyperglycemic clamp technique was performed in 107 PCOS and 20 control subjects to evaluate insulin sensitivity.      Results      The frequencies of AA

AG and GG genotypes in PCOS group were 62.59%

33.09% and 4.32% respectively

and in comparison with those of control group (60.83%

36.67% and 2.5%)

there was no significant difference (Chi-square value=0.88

P=0.64). The allele frequencies also showed no significant difference between the two groups (P=0.99). In PCOS group

the LDL-c and HDL-c of the AG/GG genotype were lower than those of AA genotype (P=0.028

P=0.036

respectively).      Conclusion      TRB3 gene +251A/G polymorphism has no correlation with PCOS in Han population in Chongqing area

but it may be correlated with dyslipidemia in this population.