少突胶质细胞相关microRNA差异性调控大脑和小脑神经前体细胞的增殖

贺雪莲; 于洋; 周永杰; 何立; 陈祥; 肖岚; 鲁青; 王凡

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少突胶质细胞相关microRNA差异性调控大脑和小脑神经前体细胞的增殖

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- 少突胶质细胞相关microRNA差异性调控大脑和小脑神经前体细胞的增殖
- Oligodendrocyte-related miRNAs differentially regulate proliferation in neural progenitor cells from mouse cerebrum and cerebellum
- 陆军军医大学学报 2011年33卷第16期页码：1667-1671
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  中图分类号： R322.81;R329.28;R394-33
- 纸质出版：2011
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贺雪莲,于洋,周永杰,何立,陈祥,肖岚,鲁青,王凡.少突胶质细胞相关microRNA差异性调控大脑和小脑神经前体细胞的增殖[J].陆军军医大学学报(原第三军医大学学报),2011,33(16):1667-1671.

　He Xuelian,Yu Yang,Zhou Yongjie,He Li,Chen Xiang,Xiao Lan,Lu Qing,Wang Fan.Oligodendrocyte-related miRNAs differentially regulate proliferation in neural progenitor cells from mouse cerebrum and cerebellum[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(16):1667-1671.
贺雪莲,于洋,周永杰,何立,陈祥,肖岚,鲁青,王凡.少突胶质细胞相关microRNA差异性调控大脑和小脑神经前体细胞的增殖[J].陆军军医大学学报(原第三军医大学学报),2011,33(16):1667-1671. DOI：

　He Xuelian,Yu Yang,Zhou Yongjie,He Li,Chen Xiang,Xiao Lan,Lu Qing,Wang Fan.Oligodendrocyte-related miRNAs differentially regulate proliferation in neural progenitor cells from mouse cerebrum and cerebellum[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(16):1667-1671. DOI：

摘要

目的探讨少突胶质细胞相关microRNA(miRNA)对中枢神经系统神经前体细胞增殖的作用。方法利用Dicer1loxp/loxp基因型小鼠和Olig1-Cre 品系小鼠杂交获得miRNA加工酶Dicer基因条件性敲除小鼠，再用免疫荧光染色和BrdU标记等技术检测这种Dicer条件性敲除小鼠大脑和小脑中神经前体细胞的增殖，以及脊髓中新生神经元的变化情况。结果 Dicer条件性敲除小鼠大脑中神经前体细胞增殖加快，而小脑中增殖细胞数目较正常小鼠减少(12%

P0.05)。此外脊髓中神经元的数目并未受到影响。结论少突胶质细胞相关miRNA对中枢神经系统的胶质细胞的发生和增殖具有时空特异性或差异性调节作用。

Abstract

Objective To investigate the effect of oligodendrocyte-related microRNAs (miRNAs) on the proliferation of neural progenitor cells in the central nerve system. Methods Heterozygous mice (Olig1-Cre) were crossed with mice (Dicer1

loxp/loxp

) to obtain crossbred mice (Olig1-Cre

+/-

; Dicer1

loxp/wt

)

and the crossbred mice were then bred with mice (Dicer1

loxp/loxp

) to produce Dicer-CKO mice (Olig1-Cre

+/-

; Dicer1

loxp/loxp

). The Dicer-CKO mice were subject to immunohistochemical assay and BrdU labeling to evaluate the proliferation in oligodendrocyte precursor cells in the cerebrum and cerebellum and the change in the neuron numb

er of the spinal cord. Results The proliferation of oligodendrocyte precursor cells was increased in the cerebral cortex but decreased in the cerebellum in the Dicer-CKO mice

while the number of neurons in the spinal cord had no change. Conclusion Oligodendrocyte-related miRNAs regulate the proliferation of neural progenitor cells differentially in the cerebrum and cerebellum.

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