Objective     To construct an eukaryotic expression vector encoding decorin (DCN) (pEGFP-N1-DCN) and determine its effect on proliferation

cell cycle

migration and invasion of human gastric cancer cell line SGC-7901 after transfection.     Methods     SGC-7901 cells were divided into 3 groups

control

pEGFP-N1 group and pEGFP-N1-DCN group. SGC-7901 cells were transfected by correspondin

g plasmids with liposome. The expressions of epidermal growth factor receptor (EGFR) and transforming growth factor-β1 (TGF-β1) were detected by Western blotting and RT-PCR

cell proliferation by MTT assay

cell cycle by flow cytometry and cell migration and invasion by Transwell chamber test.     Results     RT-PCR and Western blotting results showed that the expressions of EGFR and TGF-β

1

were decreased in pEGFP-N1-DCN group. MTT assay demonstrated that the value of D（490） of the control group

pEGFP-N1 group and pEGFP-N1-DCN group were 1.190±0.037

1.169±0.052

and 0.572±0.021 respectively

indicating that DCN made cell proliferation inhibited （P0.05）. Flow cytometric analysis showed that cell population at G

0

to G

1

stage of these 3 groups were (46.12±0.63)%

(46.07±0.82)%

and (56.36±0.92)%

and the cells in pEGFP-N1-DCN group were arrested at G

0

to G

1

stage （P0.05）. Transwell assay showed that DCN inhibits cell migration and invasion（P0.05）.     Conclusion     DCN plays a role of anti-tumor by inhibiting tumor cell proliferation

migration and invasion.