LPCES对慢性低压缺氧兔颏舌肌肌球蛋白重链和SR Ca<sup>2+</sup>摄取-释放动力学的影响

刘熙; 刘刚; 张妮; 欧娜; 张鹏

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LPCES对慢性低压缺氧兔颏舌肌肌球蛋白重链和SR Ca²⁺摄取-释放动力学的影响

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- LPCES对慢性低压缺氧兔颏舌肌肌球蛋白重链和SR Ca²⁺摄取-释放动力学的影响
- Electrical stimulation at lower physiological frequency induces myosin heavy chain isoform transformation and improves sarcoplasmic reticulum Ca²⁺ uptake/release in genioglossus of rabbits exposed to chronic hypoxia
- 陆军军医大学学报 2011年33卷第7期页码：690-694
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  中图分类号： R322.74;R337.5;R364.4
- 纸质出版：2011
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刘熙,刘刚,张妮,欧娜,张鹏.LPCES对慢性低压缺氧兔颏舌肌肌球蛋白重链和SR Ca2+摄取-释放动力学的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(07):690-694.

　Liu Xi,Liu Gang,Zhang Ni,Ou Na,Zhang Peng.Electrical stimulation at lower physiological frequency induces myosin heavy chain isoform transformation and improves sarcoplasmic reticulum Ca2+ uptake/release in genioglossus of rabbits exposed to chronic hypoxia[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(07):690-694.
刘熙,刘刚,张妮,欧娜,张鹏.LPCES对慢性低压缺氧兔颏舌肌肌球蛋白重链和SR Ca2+摄取-释放动力学的影响[J].陆军军医大学学报(原第三军医大学学报),2011,33(07):690-694. DOI：

　Liu Xi,Liu Gang,Zhang Ni,Ou Na,Zhang Peng.Electrical stimulation at lower physiological frequency induces myosin heavy chain isoform transformation and improves sarcoplasmic reticulum Ca2+ uptake/release in genioglossus of rabbits exposed to chronic hypoxia[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(07):690-694. DOI：

摘要

目的研究超低频复合生理频率慢性电刺激(lower physiological frequency chronic electrical stimulation

LPCES)对慢性低压缺氧兔颏舌肌肌球蛋白重链（myosin heavy chain，MHC）亚型表达和肌浆网（sarcoplasmic reticulum，SR）Ca

摄取-释放动力学的影响。方法成年日本大白兔24只，按随机数字表法分为4组（n=6）：A：正常对照组，B：慢性低压缺氧组，C：2.5 Hz电刺激组，D：(2.5+40)Hz电刺激组。B、C、D组兔在构建慢性低压缺氧模型后，给予C、D 2组慢性电刺激，刺激频率分别为超低频2.5 Hz和超低频复合生理频率(2.5+40)Hz。采用Western blot分析4组兔颏舌肌MHC亚型表达变化，采用荧光光度测定法测定SR Ca

摄取-释放动力学。结果 ①同正常对照组[(72.16±2.98)%

]

比较，慢性低压缺氧组[(80.45±1.26)%

]

兔颏舌肌MHCⅡa亚型比例明显增加（P0.05），MHCⅠ亚型比例明显减少[(10.86±1.26)% vs (18.61±3.19)%，P0.05

]

，SR Ca

摄取-释放速率明显下降，摄取-释放量也明显减少（P0.05）。②同慢性低压缺氧组比较，慢性电刺激后各组兔MHCⅡa和MHCⅠ亚型比例明显增加（P0.05），MHCⅡb亚型比例明显减少（P0.05），（2.5+40）Hz电刺激组较2.5 Hz电刺激组更为显著（P0.05）；SR Ca

摄取-释放速率加快，摄取-释放量增加（P0.05）

（2.5+40）Hz电刺激组较2.5 Hz电刺激组更为明显（P0.05）。同2.5 Hz电刺激组比较，(2.5+40)Hz电刺激组MHCⅡa和MHCⅠ亚型比例差异无统计学意义（P0.05）。结论慢性电刺激后慢性低压缺氧兔颏舌肌MHC呈现Ⅱb型向Ⅱa型和Ⅰ型的转变，SR Ca

摄取-释放功能增强，其中超低频复合生理频率组变化更加明显。

Abstract

Objective To identify the effect of chronic electrical stimulation at a lower physiological frequency on the expressions of myosin heavy chain (MHC) isoforms and kinetics of sarcoplasmic reticulum (SR) Ca

uptake/release in the genioglossus of rabbits exposed to chronic hypoxia. Methods Twenty-four adult rabbits were randomized into control group (A)

chronic hypoxia group (B)

2.5 Hz electrical stimulation group (C) and (2.5+40) Hz electrical stimulation group (low frequency plus physical frequency

D). After the rabbits from group B

C and D had been fed with free access to food and water in a hypoxia cabin (simulating 5 000 m altitude) in 10 h a day for 4 weeks

the rabbits in group C and D received electrical stimulation in their genioglossus at a frequency of 2.5 Hz and (2.5+40) Hz respectively in 10 h per day for 14 d

while those in group B received no electrical stimulation. Expressions of MHC isoforms in the genioglossus of rabbits in 4 groups were detected by Western blotting

and Fura-2 fluorophotometry was used to assay the kinetics changes of SR Ca

uptake-release. Results The expression level of MHCⅡa was significantly higher while that of MHCⅠ was significantly lower in group B than that in group A (P0.05). Meanwhile

the genioglossus SR Ca

uptake/release velocity in group B was significantly decreased compared with that in group A (P0.05). The expression levels of MHCⅡa and MHCⅠ in group C and D after electrical stimulation were significantly higher

while those of MHCⅡb

especially in group D

were significantly lower than those in group B (P0.05). The genioglossus SR Ca

uptake/release velocity in group C and D

especially in group D

was significantly increased compared with that in group B (P0.05). No significant difference was found in expression levels of MHCⅡa and MHCⅠ between group C and D after electrical stimulation (P0.05). Conclusion MHCⅡb in the genioglossus of rabbits with chronic hypoxia is tr

ansformed to MHCⅡa and MHCⅠ after electrical stimulation

and SR Ca

uptake/release velocity is increased

especially at a lower physiological frequency of (2.5+40) Hz.

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LPCES对慢性低压缺氧兔颏舌肌肌球蛋白重链和SR Ca2+摄取-释放动力学的影响

Electrical stimulation at lower physiological frequency induces myosin heavy chain isoform transformation and improves sarcoplasmic reticulum Ca2+ uptake/release in genioglossus of rabbits exposed to chronic hypoxia

DOI：

摘要

Abstract

关键词

Keywords

references

LPCES对慢性低压缺氧兔颏舌肌肌球蛋白重链和SR Ca²⁺摄取-释放动力学的影响

Electrical stimulation at lower physiological frequency induces myosin heavy chain isoform transformation and improves sarcoplasmic reticulum Ca²⁺ uptake/release in genioglossus of rabbits exposed to chronic hypoxia