Objective   To explore the effect of HIF-1α

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on invasive ability of HepG2 cells.    Methods   HepG2 cells were divide into blank group

control group and HIF-1α

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siRNA groups

The blank group received no intervention

and the control group and HIF-1α

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siRNA group were transfected by siRNA control and HIF-1α

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siRNA

respectively with the aid of Liposome. In 18 h later

the effect of HIF-1α

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siRNA on HepG2 cells invasive ability in vitro was measured by Boyden chamber invasive models. The expression of HIF-1α

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mRNA were measured by real-time PCR. Expression of HIF-1β were detected by Western blot analysis

and the contents of MMP-2 and MMP-9 in the supernatant was measured by ELISA.    Results   In HIF-1α

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siRNA group

the expression of HIF-1α

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mRNA were decreased by 70.0%

and contents of  MMP-2 and MMP-9 protein were increased by 37.72% and 55.46% (P0. 01)

respectively. The invasive ability of the HIF-1α

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siRNA transfected cells was increased by 71.1% (P0.01). HIF-1α

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siRNA had no effect on HIF-1β protein expression in HepG2 cells

but it increased the HIF-1β protein expression in HepG2 nucleus by 64.1%（P0.01）.    Conclusion   Silencing HIF-1α

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suppresses the invasive ability of HepG2 cells. This may be concerned with that it could inhibit the nuclear transfer of HIF-1β.