Objective   To prepare vancomycin-sol coating and investigate its bacteriostasis characteristics in vitro.   Methods   VCM20-SOL coating which contains 20 mg/ml vancomycin was prepared by acid-catalyzed tetraethylorthosilane with sol as carrier. Then a titanium alloy sheet was coated with the prepared VCM20-SOL coating with sol-gel technique

and the blank titanium alloy sheet served as the control group. Fourier transform infrared spectrum system (FT-IR) was used to analyze the surface groups of the VCM20-SOL coated titanium alloy sheet

Staphylococcus aureus (S. aureus) was employed as test bacterium. The antibacterial effect of the eluent from the experimental sheet and the control group was observed. Bacterial adhesion of the experiment and control sheets were evaluated.    Results   The silica sol had good properties in adsorption of vancomycin in vitro. The eluent of the experimental sheet obviously inhibited the growth of S. aureus when compared with that of control group. The bacteria adhered to the surface of experimental sheet was in dispersion

and the most of S. aureus were decomposed. However

the structure of the bacteria on the control group surface was normal

and they adhered to agglomerate

and were easy to form biofilm.    Conclusion   Our prepared vancomycin-sol coating has good antibacterial activity in vitro.