Objective   To explore the feasibility of constructing a tissue engineering heart valve (TEHV) using endothelial cells induced and differentiated from (bone mesenchymal stem cells， BMSCs) as seeded cells and collagen-chitosan complex as scaffold material.    Methods   Smooth muscle cell and fibroblasts were isolated and cultured from rabbit aortic wall and Fibroblast

smooth muscle cells and endothelial cell induced and differentiated from BMSCs were seeded in pre-moisture collagen-chitosan complex scaffo

ld in turn. The cell adhesion

growth

proliferation and stroma secretion were observed by using histochemistry

inverted and SEM scanning microscopy.    Results   The differented endothelial cells could adhere to

grow and proliferate on collagen-chitosan complex scaffold by HE staining  observation

and cells on TEHV surface grew to form a serial cell layer. TEHV surface was smoothd and cells grew better. SEM scanning observation showed that cell layer was intact. Endothelial cells on TEHV surface synthesized and secreted PGI

2

.    Conclusion   Smooth muscle cells

fibroblasts cultured from aortic wall and endothelial cells induced and differentiated from BMSCs can seed on collagen-chitosan complex scaffold in turn

to construct TEHV where endothelial cells can secrete PGI

2

.