Objective   To observe the effect of mangiferin on expressions of multidrug resistance-associated protein 3 (MRP3)

nuclear pregnane X receptor (PXR)

and CYP7A promoter-binding factor (CPF) in HepG2 cells.    Methods   Total RNA and membrane or nuclear protein were extracted from HepG2 cells 72 h after treated with mangiferin. Expressions of PXR and CPF at transcription and protein levels were detected by semi-quantitative RT PCR and Western blotting

respectively. HepG2 cells treated with  ursodeoxycholic acid (UDCA) and DMSO served as positive and negative controls

respectively.    Results   Mangiferin could significantly up-regulate the mRNA and protein expression of MRP3 in HepG2 cells. The mRNA and protein expression levels of MRP3 were 3-fold and 3.3-fold higher

respectively

in positive controls than in negative controls (P0.05). The effect of mangiferin was stronger than that of UDCA on the expression of MRP3 (P0.05). Mangiferin up-regulated the expression levels of PXR and CPF (1.7-fold)

mRNA of MRP3 (3.7-fold)

CPF (21-fold)

and protein of MRP3 (4.9-fold)

respectively

in HepG2 cells (P0.05).    Conclusion   Mangiferin can up-regulate the expression of MRP3 in HepG2 cells

which may be related with PXR and PXR.