Objective   To amplify the full-length sequences of diffuse large B cell lymphoma (DLBCL)-associated antigen-specific TCR α and β genes and to predict three-dimensional (3-D) structure of the proteins.   Methods   The full-length TCR genes were amplified from the peripheral blood mononuclear cells (PBMCs) derived from 2 pathologically identified DLBCL patients for the TCR Vα6 and Vβ13 subfamily T-cell clones

and their amino acid sequences were deduced by primer premier 5.0 analytical software. 3-D spatial structures of the TCR α and β were modeled by SWISS-MODEL

then displayed and analyzed by the analytical software of Rasmol.   Results   The full-length TCR genes and their amino acid sequences were obtained successfully. the TCR Vα6 chains from the 2 cases of DLBCL patients were of high homology not only in sequence (92.16% and 91.71%

respectively)

but also in the 3-D spatial structures

which displayed the same model (PDB ID:3ffcD) by the SWISS-MODEL homology-modeling server. While the TCR Vβ13 chains also used an identical model (PDB ID:3dx9D)

and sequence homology was 92.95% and 92.18%

respectively.   Conclusion   The TCR of Vα6 or Vβ13 subfamily T cell clones have high homology in the 3-D spatial structures from the PBMCs of 2 cases of DLBCL patients

suggesting that they might recognize similar tumor associated antigen epitope.