Objective   To investigate the effect of integrinβ

1

gene on uptaking ox-LDL in RAW264.7 cells.    Methods   Three siRNAs were designed and synthesized according to different targets on integrinβ

1

gene

and then transfected into RAW264.7 cells by lipidosome. RAW264.7 cells were divided into control (without transfection)

siRNANC (with transfection of siRNA negative)

lipo2000 (without transfection but added lipo2000)

siRNA (with transfection of effective siRNA). siRNA labeled by Cy3 was used for transfection efficiency detecti

on

and real-time PCR was used for siRNA with highest silence efficiency screening. The change of cell adhesion was analyzed by cell adhesion experiment

and cell uptaking ox-LDL was evaluated by formation of lipid granules in the cells with oil red staining.    Results   siRNA integrinβ

1

was successfully transfected into RAW264.7 cells with a highest silence efficiency of 65%. The expression of integrinβ

1

mRNA was obviously decreased in siRNA1 group

in which cell adhesion was decrease form 70% to 53% as well as the foam cell formation was decreased from 90% to 17%. Statistical analysis showed the siRNA1 group had significant difference in ox-LDL uptake compared with the other groups (P0.05).    Conclusion   Silencing integrinβ

1

gene decreases  the function of uptaking ox-LDL in RAW264.7 cells

and then inhibits the cells to transform into foam cells.