Objective   To investigate the effect of curcumin derivant (hydrazinocurcumin

HC) on the invasion and migration of human breast cancer of MDA-MB-231 cells through inhibiting STAT3 signal pathway.    Methods   Human breast cancer MDA-MB-231 cells were divided into 3 groups

control group，10 and 20 μmol/L HC treatment groups. Growth inhibition was measured with MTT assay. Morphological changes were detected by Liu staining. Cell invasion was measured by Transwell chamber

and migration of the cells was measured with scratch assay. Expressions of STAT3

p-STAT3

MMP-9

and MMP-2 were detected by Western blot analysis.    Results   After treated with HC

the proliferation of MDA-MB-231 cells was inhibited in a time and dosedepending manner. Liu staining demonstrated that the cell morphology had been changed

and became round in shape. The invasion assay showed the number of treated MDA-MB-231 cells was lower than those of control (94.00±6.56 and 4.00±1.00 vs 598.00±21.28

P0.05). Scratch assay also showed the treated cells were inhibited. Western blot analysis demonstrated that the expressions of p-STAT3

MMP-9 and MMP-2 were decreased

while total STAT3 had no significant difference compared with control.    Conclusion   HC inhibits the invasion and migration of the human breast cancer of MDA-MB-231 cells

probably by inhibiting STAT3 activity

and then reducing MMP-9 and MMP-2 expressions.