Objective   To establish the allergic trachitis model of mice with Humulus pollen allergen infusion.  Methods   Humulus pollen allergen infusion was prepared. Forty clean and female BALB/c mice were randomly divided into blank control group sensitized with PBS+ aluminium hydroxide

low dose group sensitized with 100 μg Humulus pollen allergen infusion + aluminium hydroxide

medium dose group sensitized with 200 μg Humulus pollen allergen infusion + aluminium hydroxide

and high dose group sensitized with 300 μg Humulus pollen allergen infusion + aluminium hydroxide

10 mice in each g

roup.After lung tissue samples from mice were stained with haematoxylin and eosin(HE)

inflammation and mucus secretion were observed

total number of cells in alveoli lavage solution and their composition were calculated

BALF

IL-4

IFN-γ

IgE and IgG1 in homogenate supernatant of splenocytes were assayed by enzyme-linked immunoassay (ELISA).  Results   Allergic inflammation aggravated with the increasing concentration of allergen. The total number of cells

macrophages

and EOS in alveoli lavage solution was (210.75±78.80)×10

6

/L

48.65%±3.27%

and 24.37%±4.28%

respectively. The serum levels of IL-4

IgG1 and IgE were significantly higher in low

medium and high dose groups than in blank control group (P0.05). However

the IFN-γ level in alveoli lavage solution and homogenate supernatant of splenocytes was lower in low

medium and high dose groups than in blank control group (P0.05).  Conclusion   Humulus pollen allergen infusion can be used in establishing the allergic trachitis model of mice.