Objective    To compare and analyze the difference of γ-tubulin and aurora-A expression in human cervical cancer cells (CasKi ) and immortalized human cervical squamous H8 cells with positive HPV16 E6E7.     Methods    Difference of γ-tubulin and aurora-A expression in CasKi and H8 cells was analyzed by showing the fluorescence intensity of γ-tubulin with indirect immunofluorescence. Expression level of aurora-A mRNA was detected by RT-PCR. Expression level of γ-tubulin and aurora-A in CasKi and H8 cells was semi-quantitatively analyzed by Western blot.     Results    The immunofluorescence signal of γtubulin was stronger in Caski cells  than in H8 cells (57.78±3.13 vs 37.37±2.37

P0.05). The expression level of aurora-A mRNA was significantly higher in CasKi cells than in H8 cells (0.745±0.013 vs 0.617±0.223

P0.05). Western blot showed that the expression level of γ-tubulin and aurora-A was significantly higher in CasKi cells than in H8 cells (0.969±0.011 and 0.909±0.019 vs 0.568±0.016 and 0.571±0.022

P0.05).     Conclusion    γ-tubulin and aurora-A are over-expressed in CasKi and H8 cells

more significant CasKi cells

suggesting that amplification and over-expression of CasKi cells can be used as complementary biological indices in diagnosis of cervical epithelial cancer.