Objective　To prepare the human anti-HIF-1α scFv of lung adenocarcinoma from phage antibody library and to study the radioimmunoimaging (RII) of scFv in nude mice xenografted with A549 tumor cells in vivo.　Methods　Specific anti-HIF-1α scFv of lung adenocarcinoma was selected from the phage antibody library. Expression of soluble scFv was induced by infecting E.coli HB2151.

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I-labeled scFv was injected into the tail veins of nude mice xenografted with A549 tumor cells. Static imaging was

performed by SPECT 24

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72 and 120 h after injection of soluble scFv to observe the radioactivity in tumor. Radioactivity ratio of tumor and non-tumor (T/NT) was assayed using the ROI technique. When the tumor was clearly visible

image fusion was performed by SPECT/CT.　Results　The specific human anti-HIF-1α scFv of lung adenocarcinoma was successfully prepared. The RII showed that the

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I-labeled scFv was selectively accumulated in lung adenocarcinoma tissue. Radioisotope uptake and T/NT reached their peak value of 3.19 in the tumor and the image was well fused with the tumor clearly seen 72 h after injection of soluble scFv.　Conclusion　Human anti-HIF-1α scFv of lung adenocarcinoma can be successfully prepared.

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I-labeled scFv can clearly show the tumor on RII in nude mice xenografted with A549 tumor cells

thus laying a foundation for further study on the diagnosis and treatment of lung adenocarcinoma.