Objective　To study the inhibitory effect of small interfering RNA (siRNA) targeting heat shock protein 90 beta (Hsp90β) gene on growth of Jurkat cells.　Methods　Three specific interference sequences and a random controlled sequence were inserted into the pSOS-HUS according to the cDNA sequence Hsp90B1（NM_003299.1）of Hsp90β gene. Recombinant eukaryotic expression plasmids

including pSOS-Hsp90βi1

pSOS-Hsp90βi2

pSOS-Hsp90βi3 and pSOS-Hsp90βi control

were constructed and transfected into Jurkat cells by LipofectamineTM LTX. Gene silencing efficiency of recombinant plasmid pSOS-Hsp90βi was monitored by real-time PCR and effective Hsp90β-specific RNAi sequences were screened. Hsp90β expression level was measured by Western blotting. Inhibitory effect of Hsp90β gene on growth of Jurkat cells was detected by MTT assay and flow cytometry

respectively

before and after interfered by siRNA.　Results　The recombinant plasmid pSOS-Hsp90βi1

2

3 eukaryotic vector targeting Hsp90β was successfully constructed. The expression of Hsp90β in Jurkat cells transfected with pSOS-Hsp90βi2 was significantly inhibited at both mRNA and protein levels (P0.05). MTT assay and flow cytometry showed that Hsp90βsiRNA inhibited the proliferation of Jurkat cell (P0.01) 19.56% of Jurkat cells apoptosis were also observed (P0.05).　Conclusion　Chemically synthesized specific siRNA targeting Hsp90β can effectively reduce the expression of Hsp90β gene and growth of leukemia Jurkat cells.