应用组织芯片技术研究胃癌组织MVD、MLD和PTEN的表达及其意义

邹绍静; 周永宁; 姬瑞; 路红; 郭庆红; 王娟霞; 任涛文; 陈兆峰; 黄珊珊; 张俐花; 赵越

您当前的位置：

首页 >

文章列表页 >

应用组织芯片技术研究胃癌组织MVD、MLD和PTEN的表达及其意义

论著 | 更新时间：2026-01-09

- 应用组织芯片技术研究胃癌组织MVD、MLD和PTEN的表达及其意义
- Significances of microvessel density, microlymphatic density and PTEN protein expressions in gastric cancer by tissue microarray
- 陆军军医大学学报 2010年32卷第18期页码：1936-1940
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R446.9;R730.23;R735.2
- 纸质出版：2010
- 稿件说明：
移动端阅览
邹绍静,周永宁,姬瑞,路红,郭庆红,王娟霞,任涛文,陈兆峰,黄珊珊,张俐花,赵越.应用组织芯片技术研究胃癌组织MVD、MLD和PTEN的表达及其意义[J].陆军军医大学学报(原第三军医大学学报),2010,32(18):1936-1940.

　Zou Shaojing,Zhou Yongning,Ji Rui,Lu Hong,Guo Qinghong,Wang Juanxia,Ren Taowen,Chen Zhaofeng,Huang Shanshan,Zhang Lihua,Zhao Yue.Significances of microvessel density, microlymphatic density and PTEN protein expressions in gastric cancer by tissue microarray[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(18):1936-1940.
邹绍静,周永宁,姬瑞,路红,郭庆红,王娟霞,任涛文,陈兆峰,黄珊珊,张俐花,赵越.应用组织芯片技术研究胃癌组织MVD、MLD和PTEN的表达及其意义[J].陆军军医大学学报(原第三军医大学学报),2010,32(18):1936-1940. DOI：

　Zou Shaojing,Zhou Yongning,Ji Rui,Lu Hong,Guo Qinghong,Wang Juanxia,Ren Taowen,Chen Zhaofeng,Huang Shanshan,Zhang Lihua,Zhao Yue.Significances of microvessel density, microlymphatic density and PTEN protein expressions in gastric cancer by tissue microarray[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(18):1936-1940. DOI：

摘要

目的探讨PTEN在胃癌、癌旁组织中表达及微血管密度（microvessel density

MVD）、微淋巴管密度(microlymphatic density

MLD)，分析其表达水平与临床病理特征和生存期的关系。方法用组织芯片和免疫组化的方法检测189例胃癌组织、54例癌旁组织、32例正常胃黏膜中PTEN的表达及微血管密度、微淋巴管密度。结果胃癌、癌旁和正常胃黏膜组织PTEN阳性表达率分别是50.8%（96/189）、85.2%（46/54）和96.9%（31/32）

胃癌组织PTEN阳性表达率显著低于癌旁和正常胃黏膜组织（χ

=19.330，P=0.000；χ

=22.830，P=0.000）。PTEN阳性表达与有无淋巴结转移、分化程度和Lauren分型有关（P0.05）。胃癌组织MVD和MLD分别显著高于癌旁组织和正常胃黏膜组织[MVD: （28.84±14.17）vs（17.02±8.54)、（16.69±7.21）；MLD: （8.55±4.98）vs (4.05±2.48)、（3.99±1.56）；P0.01

]

。微血管密度与分化程度、Lauren分型及浸润深度密切相关（P0.01）；微淋巴管密度则与分化程度、Lauren分型及淋巴结转移密切相关（P0.05）；微血管密度、微淋巴管密度在PTEN阴性表达组显著高于阳性表达组[MVD: （31.37±15.14）vs （26.86±13.29）

P=0.031；MLD: （9.37±4.23）vs（7.61±5.29）

P=0.012

]

。PTEN的表达和MVD（r=-0.168，P=0.021）、MLD（r=-0.283，P=0.000）呈负相关关系。生存分析显示：低MVD较高MVD具有生存优势（χ

=4.224，P=0.040），MLD与生存无相关性（χ

=1.044，P=0.307），PTEN阳性表达具有显著的生存优势(P0.05)，是独立于胃癌分期、分级和分型的生存期预测因子。结论 PTEN低表达参与胃癌的发生、发展和浸润转移，是胃癌发生的晚期事件。失表达的PTEN可增加胃癌组织MVD、MLD 。PTEN的表达可以作为胃癌预后的独立指标。

Abstract

Objective To investigate the expressions of PTEN (gene for phophatase and tensin homolog deletedon chromosome ten)

and changes of microvessel density (MVD) and microlymphatic density (MLD) in gastric cancer

para-cancerous and normal tissues

and analyze their relationship with tumor clinicopathological features and patient survival. Methods Tissue microarrays containing gastric normal gastric mucosa (n=32)

para-cancerous tissue (n=54) and gastric cancer (n=189) were prepared and the expression of PTEN was detected by immunohistochemistry. Moreover

MVD and MLD were evaluated within the microarrays by double immunohistrchemistry staining with Ⅷ factor polyclonal antibodies and a D2-40 antibody

respectively. Clinical data were retrospectively analyzed. Results The positive rates for PTEN of the gastric carcinoma

para-cancerous and normal tissues were respectively 50.8% (96/189)

85.2% (46/54) and 96.9% (31/32). The positive rate of PTEN was lowest in the gastric carcinoma among the 3 groups (Chi square=19.330

P=0.000; Chi square=22.830

P=0.000). In gastric cancer tissues

the positive rates of PTEN protein were significantly associated with differentiation

lymph node metastasis

Lauren’s classification (P0.05)

and not associated with sex

age

invasion

and tumor size (P0.05). MVD and MLD were significantly higher in gastric carcinoma than in para-cancerous as well as normal tissues (MVD: 28.84±14.17 vs 17.02±8.54 and 16.69±7.21; MLD: 8.55±4.98 vs 4.05±2.48 and 3.99±1.56

P0.01). MVD was significantly correlated with tumor differentiation

Lauren’s classification and invasion (P0.01). Meanwhile

MLD was significantly correlated with tumor differentiation

Lauren’s classification and lymph node metastasis (P0.05). MVD and MLD were significantly lower in PTEN-positive tumors compared with PTEN-negative tumors (MVD: 31.37±15.14 vs 26.86±13.29

P=0.031; MLD: 9.37±4.23 vs 7.61±5.29

P=0.012). The positive rate of PTEN in the gastric carcinoma were negative correlated with MVD and MLD (r=-0.168

P=0.021; r=-0.283

P=0.000). A survival advantage were noted in the patients with PTEN-positive tumors and lower MVD tumors (P0.05)

but MLD had no such correlation (Chi square=1.044

P=0.307). The positive rates of PTEN protein was an independent prognostic index of type

grade

and stage of the disease (P0.05). Conclusion The lower-expression of PTEN is involved in the incidence

development

differentiation

invasion

and metastasis of gastric cancer

and a late incident in gastric cancer. The lower-expression of PTEN can enhance MVD and MLD in gastric cancer. PTEN may be a novel prognostic marker of gastric cancer.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

弥散加权磁共振成像评价大鼠胶质瘤新生血管的价值

茶氨酸对内皮细胞生长及肺癌血管生成的影响

超声光散射断层成像与乳腺癌HIF-1α表达的相关性

大肠癌淋巴管生成与β-连环素异位表达的关系

胃癌组织中CEACAM6、MMP-9的表达及其与临床病理的关系