Objective　　To observe the protection against ischemia preconditioning-induced vascular reactivity and calcium sensitivity after hemorrhagic shock. 　　Methods　　Effects of protein kinase Cα (PKCα) and protein kinase Cε(PKCε) antagonists on ischemia preconditioning-induced vascular reactivity and calcium sensitivity were observed in rat model after different volumes of blood loss (2.5%

5% and 10%) at different time points (0.5

1

2 and 3 h) before shock. Effects of ischemia preconditioning on translocation of PKCα and ε in superior mesenteric artery (SMA) were also observed. 　　Results　　 Ischemia preconditioning with 5% blood volume 30 min before shock improved the decreased blood pressure elevation and vasoconstriction responses to norepinephrine (NE)

and the vascular reactivity and calcium sensitivity were decreased 2 h after shock by 25.5%

25.0%

42.3% and 40.9% respectively (P0.01). The inhibitor of PKCα and ε suppressed the protective effects of ischemia preconditioning on vascular reactivity by 45.0% and 72.4% respectively (P0.01) and calcium sensitivity by 59.0% and 52.5% respectively (P0.01). Ischemia preconditioning promoted the translocation of PKCα and PKCε from cytoplasm to membrane (P0.01). 　　Conclusion　　Ischemia preconditioning can protect vascular reactivity and calcium sensitivity after hemorrhagic shock in rats through PKCα and PKCε.