Objective  To study the effect of evodiamine (EVO)-stimulated dendritic cells (DC) of spinal cord homogenate protein (hpDC) on repair of spinal cord injury (SCI) in mice.   Methods  Twenty hours after SCI

mice were randomly divided into 6 groups and injected with PBS

EVO

DC

DC+EVO

hpDC and hpDC+EVO. BMS and histopathology were used to observe the effect of hpDC+EVO on the functional recovery of spinal cord injury and pathological changes such as local scars. Levels of brain-derived neurotrophic factor (BDNF)

neurotrophin-3 (NT-3)

interleukin-12 (IL-12) and granulocyte macrophage colony stimulating factor (GM-CSF) in injured sites and supernatant of cultured T cells were measured by ELISA.   Results  Eighty-four days after treatment

the BMS was significantly higher in hpDC + EVO group than in other groups（P0.05）. Histopathology showed that the cavities and scars were smaller in hpDC group than in other groups. The levels of BDNF

NT-3

GM-CSF

and IL-12 in the injured sites were significantly higher in hpDC + EVO group than in other groups（P0.05）. The levels of BDNF and GM-CSF in supernatant of cultured T cells were significantly higher in hpDC + EVO group than in hpDC group（P0.05）.    Conclusion  hpDC+ EVO promotes the repair of SCI possibly by up-regulating the BDNF

NT-3

GM-CSF

IL-12 in injured sites.