Objective　To investigate the dynamical changes of  interleukin-1 receptor-associated kinases-1 (IRAK-1) mRNA and protein

and to explore the relationship between these changes and concentrations of TNF-α in rat B35 neurons under hypoxia.  　Methods　B35 cells exposed to hypoxia of 3%O

2

5%CO

2

and 92%N

2

were cultured for

1

3

6

12

24

48 and 72 h respectively

the mRNA and protein expressions of IRAK-1 were detected by RT-PCR and Western blot analysis

the distribution of IRAK-1 in the cells were observed by laser scanning confocal microscopy (LSCM)

and the concentrations of TNF-α were measured by ELISA.  　Results　The mRNA and protein expressions of IRAK-1 were increased after hypoxia for 1 h and decreased gradually after reaching the highest level at 6 h (P0.05)

so did the   fluorescence intensity under LSCM and the concentrations of TNF-α. After 48 h

the concentrations of mRNA and protein were decreased lower than that in normoxia (P0.05). The concentrations of TNF-α were correlated positively with the expression of IRAK-1 protein (r=0.88

P0.05). 　Conclusion　Hypoxia up-regulates the mRNA and protein of IRAK-1

which are correlated positively with the expression of TNF-α in rat B35 neurons.