建立禽流感H7和H9亚型荧光定量PCR检测方法

王秋泉; 黄平; 胡守旺; 高秀洁; 杨杏芬; 李杰

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建立禽流感H7和H9亚型荧光定量PCR检测方法

论著 | 更新时间：2026-01-09

- 建立禽流感H7和H9亚型荧光定量PCR检测方法
- Establishment of fluorescence quantitative PCR method for detection of avian influenza virus subtypes H7 and H9
- 陆军军医大学学报 2010年32卷第3期页码：246-249
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  中图分类号： R373.13;R394-33;R446.5
- 纸质出版：2010
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王秋泉,黄平,胡守旺,高秀洁,杨杏芬,李杰.建立禽流感H7和H9亚型荧光定量PCR检测方法[J].陆军军医大学学报(原第三军医大学学报),2010,32(03):246-249.

　Wang Qiuquan,Huang Ping,Hu Shouwang,Gao Xiujie,Yang Xingfen,Li Jie.Establishment of fluorescence quantitative PCR method for detection of avian influenza virus subtypes H7 and H9[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(03):246-249.
王秋泉,黄平,胡守旺,高秀洁,杨杏芬,李杰.建立禽流感H7和H9亚型荧光定量PCR检测方法[J].陆军军医大学学报(原第三军医大学学报),2010,32(03):246-249. DOI：

　Wang Qiuquan,Huang Ping,Hu Shouwang,Gao Xiujie,Yang Xingfen,Li Jie.Establishment of fluorescence quantitative PCR method for detection of avian influenza virus subtypes H7 and H9[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(03):246-249. DOI：

摘要

目的建立高致病性禽流感H7和H9亚型荧光定量PCR方法。方法根据禽流感病毒(avian influenza virus，AIV)HA-H7基因和HA-H9基因分别设计特异AIV H7和H9亚型特异荧光定量PCR引物和探针，优化反应体系和反应条件，分析该试剂的敏感性、特异性、重复性等。结果经优化反应体系和反应条件后，AIV H7试剂和AIV H9试剂的敏感性达到1×10

4.67

EID

和1×10

3.80

EID

水平，特异性与细胞培养敏感性类似；批内和批间精密度Ct值的CV值均远小于10%，可重复性良好。结论本项目研制的AIV H7和H9亚型的敏感性、特异性、重复性均好，适合在禽流感H7和H9亚型疫情和人类疑似禽流感病例中检测使用。

Abstract

Objective To establish the fluorescence quantitative PCR method for detection of avian influenza virus (AIV) subtypes H7 and H9. Methods Primers and probe of fluorescence quantitative PCR were designed for AIV subtypes H7 and H9

respectively

according to the AIV HA H7 and H9 genes. The reaction system and conditions were optimized. Sensitivity

specificity and reproducibility of the system were analyzed. Results After optimization of the reaction system and its conditions

the sensitivity of H7 and H9 assay reached 1 10

4.67

EID

and 1 10

3.80

EID

respectively

and their specificity was similar to that of cell cu

lture. The CV values of CT in intra-and inter-batch were far less than 10%

showing that their reproducibility was excellent. Conclusion The sensitivity

specificity and reproducibility of AIV subtypes H7 and H9 are rather good. AIV subtypes H7 and H9 can be used in detection of avian epidemic in suspected avian H7 and H9 infected human cases.

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