Objective  To investigate whether bone marrow cells (BMC) transfected with multidrug resistance gene-1 (mdr-1) can tolerate to the killing effect of overdose mycophenolate mofetil (MMF).   Methods  BMC were transfected with recombinant adenovirus

Ad-EGFP-mdr-1. Expression levels of mdr1 mRNA and protein were detected by RT-PCR

Western blotting and daunorubicin (DAM)

respectively. The survival of mdr-1 transfected BMC after treatment with MMF at different concentrations was detected by MTT.   Results  The transfection efficiency of the virus was 30.14%

and the survival rate of BMC was 89.25%. The mdr-1 mRNA and protein were functionally expressed in transfected BMC. The expressed product

P-gp

was found to have a pumping function induced by mdr-1. No obvious difference was found in survival of mdr-1 transfected BMC (P0.05).   Conclusion  mdr-1 transfected MBC can efficiently tolerate to overdose MMF

thus providing a basis of its in vivo experiments.