Objective    To study the role of parathyroid hormone-related peptide (PTHrP) in transdifferentiation of NRK-52E cells induced by high glucose.     Methods    Expression of PTHrP and its receptor 1 in NRK-52E cells incubated with 0 (control group)

5 (normal glucose group) and 16.7mmol/L glucose (high glucose group)

respectively

was detected by RT-PCR and Western blotting

respectively. Expression of TGF-β1

MMP2

type Ⅰ collagen and α-SMA in NRK-52E cells of normal and high glucose groups was detected by Western blotting and ROS was detected by CM2H2DCFDA after 72 h.      Results    The expression levels of PTHrP

PTHrP receptor 1 (PTH1R)

and PTHrP mRNA were higher in high glucose group than in control and normal glucose groups (P0.05). The expression of TGF-β1

type Ⅰ collagen

and α-SMA after interfered with PTHrP was significantly higher in normal and high glucose groups than in control group (P0.05). The expression of MMP2 was up-regulated after interference with PTHrP and high glucose level (P0.01). The PTHrP and high glucose level increased the ROS in NRK-52E cells (P0.01).     Conclusion    High glucose level can influence the transdifferentiation of NRK-52E cells by regulating PTHrP and its receptor level.