IL-15诱导CD4+CD25- T细胞向CD4+CD25+调节性T细胞转化的机制探讨

徐述雄; 傅晓岚; 孙兆林; 李晓伟; 陈丽萍; 刘宏; 吴雄飞

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IL-15诱导CD4⁺CD25^-T细胞向CD4⁺CD25⁺调节性T细胞转化的机制探讨

论著 | 更新时间：2026-01-09

- IL-15诱导CD4⁺CD25^-T细胞向CD4⁺CD25⁺调节性T细胞转化的机制探讨
- IL-15 converts CD4⁺CD25^- T cells into CD4⁺CD25⁺ regulatory T cells
- 陆军军医大学学报 2010年32卷第8期页码：759-762
- 作者机构：
- 作者简介：
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  中图分类号： R392-33;R392.11;R392.2
- 纸质出版：2010
- 稿件说明：
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徐述雄,傅晓岚,孙兆林,李晓伟,陈丽萍,刘宏,吴雄飞.IL-15诱导CD4+CD25- T细胞向CD4+CD25+调节性T细胞转化的机制探讨[J].陆军军医大学学报(原第三军医大学学报),2010,32(08):759-762.

　Xu Shuxiong,Fu Xiaolan,Sun Zhaolin,Li Xiaowei,Chen Liping,Liu Hong,Wu Xiongfei.IL-15 converts CD4+CD25- T cells into CD4+CD25+ regulatory T cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(08):759-762.
徐述雄,傅晓岚,孙兆林,李晓伟,陈丽萍,刘宏,吴雄飞.IL-15诱导CD4+CD25- T细胞向CD4+CD25+调节性T细胞转化的机制探讨[J].陆军军医大学学报(原第三军医大学学报),2010,32(08):759-762. DOI：

　Xu Shuxiong,Fu Xiaolan,Sun Zhaolin,Li Xiaowei,Chen Liping,Liu Hong,Wu Xiongfei.IL-15 converts CD4+CD25- T cells into CD4+CD25+ regulatory T cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(08):759-762. DOI：

摘要

目的　初步探讨IL-15诱导CD4

CD25

T细胞向CD4

CD25

调节性T细胞（Tregs）转化的机制。　方法　采用免疫磁性细胞分离法分离人外周血中的CD4

CD25

T细胞，依据加入细胞因子不同分为4组：对照组、IL-15组、拮抗1组、拮抗2组，其中后3组均于培养的第1天加入IL-15，而拮抗1组和拮抗2组分别于培养第1、3天加入STAT

封闭性肽。流式细胞仪检测细胞表型变化，

H-TdR掺入法检测IL-15诱导产生的Tregs抑制CD4

CD25

效应T细胞（Teff）增殖的免疫功能，Western blot法检测细胞内p-STAT

的变化。　结果　与对照组比较，IL-15能诱导CD4

CD25

T细胞中CD25和Foxp3表达上调，IL-15诱导生成的Tregs具有较天然Tregs稍弱的免疫抑制功能。Western blot检测结果显示，IL-15组p-STAT

表达较对照组明显上调。加入STAT

封闭性肽后，拮抗1组和拮抗2组中CD4

CD25

T细胞中CD25和Foxp3表达下调，但拮抗2组[细胞表型分别为(47.9±6.0)%、（20.7±3.4）%

]

改变较拮抗1组[细胞表型分别为（30.7±8.5）%、（9.2±2.2）%

]

改变轻微。　结论　IL-15能诱导CD4

CD25

T细胞向Tregs转化，其机制可能是上调p-STAT

的表达。

Abstract

Objective　To investigate the effect and mechanism of IL-15 converting CD4

CD25

T cells into CD4

CD25

regulatory T cells (Tregs).

　Methods　CD4

CD25

T cells were isolated from human peripheral blood mononuclear cells in 2 steps by magnetic cell sorting system. The purity rate of the sorted cells was measured by flow cytometry (FCM). CD4

CD25

T cells were divided into 4 groups: control group (without cytokines)

IL-15 group (IL-15)

antagonism I group (IL-15 and STAT

blocking peptide was added at the first day) and Antagonism Ⅱ group (IL-15 was added at the first day

but STAT

blocking peptide at the third day). FCM was used to detect the changes of CD4

CD25

T cells

the incorporation of [

]

thymidine was used to evaluate the suppressive function of the IL-15-induced Tregs (iTregs) on the Teff and Western blot analysis was used to observe the expression of p-STAT

. 　Results　Compared with CD4

CD25

T cells cultured without cytokines

IL-15 was able to promote CD25 and Foxp3 expression of CD4

CD25

T cells and IL-15induced CD4

CD25

regulatory T cells exerted weak suppressor activity. Addition of IL-15 up-regulated the expression of p-STAT

in CD4

CD25

T cells significantly. After STAT

blocking peptide was added

IL-15-induced CD25 and Foxp3 expression of CD4

CD25

T cells was significantly down-regulated. 　Conclusion　IL-15 significantly converts naive CD4

CD25

T cells into CD4

CD25

regulatory T cells in vitro and up-regulated p-STAT

may be involved in this process.

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