黄芪、当归对血管内皮细胞纤溶酶原激活物抑制剂-1的影响

杨长春; 马增春

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黄芪、当归对血管内皮细胞纤溶酶原激活物抑制剂-1的影响

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- 黄芪、当归对血管内皮细胞纤溶酶原激活物抑制剂-1的影响
- Effect of Astragalus Membranaceus and Angelica Sinensis on plasminogen activator inhibitor-1 in cultured endothelial cells
- 陆军军医大学学报 2010年32卷第11期页码：1149-1151
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  中图分类号： R282.71;R364.15;R392.2
- 纸质出版：2010
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杨长春,马增春.黄芪、当归对血管内皮细胞纤溶酶原激活物抑制剂-1的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(11):1149-1151.

　Yang Changchun,Ma Zengchun.Effect of Astragalus Membranaceus and Angelica Sinensis on plasminogen activator inhibitor-1 in cultured endothelial cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(11):1149-1151.
杨长春,马增春.黄芪、当归对血管内皮细胞纤溶酶原激活物抑制剂-1的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(11):1149-1151. DOI：

　Yang Changchun,Ma Zengchun.Effect of Astragalus Membranaceus and Angelica Sinensis on plasminogen activator inhibitor-1 in cultured endothelial cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(11):1149-1151. DOI：

摘要

目的观察中药黄芪、当归对血管内皮细胞纤溶酶原激活物抑制剂-1（plasminogen activator inhibitor-1，PAI-1）表达及活性的影响，探讨黄芪、当归抗血栓作用的分子机制。方法使用成品的黄芪和当归注射液，各设两个浓度（6 mg/ml和 3 mg/ml），分别加入细胞培养板，实验组各孔均加入0.5 ng/ml TGF-β1作为诱导剂以增加PAI-1Ag表达。同时设立TGF-β1组（细胞液中只加入0.5 ng/ml TGF-β1）以及空白对照组（细胞液中不加入任何成分），各组在37 ℃，5%CO

孵箱中培养24 h。应用RT-PCR方法检测黄芪、当归对血管内皮细胞PAI-1 mRNA表达的影响，ELISA法检测黄芪、当归对PAI-1表达的影响，发色底物显色法检测黄芪、当归对PAI-1活性的影响。结果黄芪、当归可不同程度地降低血管内皮细胞PAI-1 mRNA表达、抗原水平与活性，以黄芪、当归联合应用作用更为明显。黄芪、当归单独及联合应用对PAI-1 mRNA表达的抑制率分别为17%、24.6%和36.6%。结论黄芪、当归可能通过抑制血管内皮细胞PAI-1表达和活性而发挥其抗血栓形成的作用。

Abstract

Objective To investigate the effect of Astragalus Membranaceus(Ast) and Angelica Sinensis(Ang) on antithrombosis and explore the molecular mechanism. Methods Plasminogen activator inhibitor-1(PAI-1) expression and activity were selected to observe the antithrombosis effect of Ast and Ang (3 and 6 mg/ml) on 0.5 ng/ml TGF-β

treated endothelial cells. PAI-1mRNA expression was detected by RT-PCR

PAI-1 antigen assay was detected by specific enzyme-linked immunosorbent assays(ELISA)

and PAI-1 activity was measured by amidolytical assay. Results Ast and Ang significantly inhibited PAI-1 mRNA expreesion

antigen and activity in endothelial cells. The more powerful effects could be observed when treated by Ast and Ang together. Conclusion The PAI-1 mRNA expression，antigen and activity is inhibited effectively by Ast and Ang

which may be the mechanism of their treatment for antithrombosis.

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